小肠结肠炎耶尔森菌O:8血清型特异性单克隆抗体的制备

目的 利用杂交瘤技术制备小肠结肠炎耶尔森菌O:8血清型特异性单克隆抗体,用于小肠结肠炎耶尔森菌O:8血清型菌株的分型鉴定.方法 用小肠结肠炎耶尔森菌O:8血清型菌株作为抗原,以骨髓杂交瘤细胞融合技术制备小肠结肠炎耶尔森单克隆抗体,用玻片凝集筛选法进行单克隆抗体的特异性鉴定.结果 筛选到1株与小肠结肠炎耶尔森菌O:8血清型菌株呈现阳性反应,并与日本的抗血清检测结果相一致,而与被检测的其他血清型小肠结肠炎耶尔森菌和常见肠道致病菌无交叉凝集反应.结论 本实验筛选获得的单克隆抗体,用于小肠结肠炎耶尔森菌O:8血清型菌株鉴定,具有良好的特异性,避免了免疫血清难以避免的交叉凝集现象.

Preparation of Yersinia enterocolitica serotype O:8-specific monoclonal antibody

Objective The hybridoma technology was used in this study to prepare Yersinia enterocolitica serotype O:8-specific monoclonal antibodies for typing and identification. Methods Yersinia enterocolitica serotype O:8 strains were the antigens used in the bone marrow hybridoma technology for the preparation of Yersinia enterocolitica monoclonal antibodies. Slide agglutination screening was conducted to identify the specificity of monoclonal antibodies. Results Positive reactions to Yersinia enterocolitica serotype O:8 strains were detected in one group of the antibodies, consistent with previous antiserum test results in Japan. In addition, cross agglutination to other serotypes of Yersinia enterocolitica and common intestinal bacteria was absent in this group of antibodies. Conclusion The group of monoclonal antibodies obtained in the screening was highly specific to Yersinia enterocolitica serotype O:8 strains without cross agglutination problems commonly seen in immune serum. Hence, these antibodies would be conducive to the identification of Yersinia enterocolitica serotype O:8 strains.