| Cocktail法评价白香丹胶囊对大鼠体内CYP450酶活性的影响 |
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| 引用本文： | 孙冰婷,张倩,宗阳,何书芬,朱立静,居文政. Cocktail法评价白香丹胶囊对大鼠体内CYP450酶活性的影响[J]. 药学与临床研究, 2016, 24(1) |
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| 作者姓名： | 孙冰婷 张倩 宗阳 何书芬 朱立静 居文政 |
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| 作者单位： | 南京中医药大学附属医院临床药理科,南京中医药大学附属医院临床药理科,南京中医药大学附属医院临床药理科,南京中医药大学附属医院临床药理科,南京中医药大学附属医院临床药理科,南京中医药大学附属医院临床药理科 |
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| 基金项目： | 国家自然科学基金项目(81573685)；江苏高校优势学科建设工程资助项目(2010)；重大新药创新(2012ZX09303009-002) |
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| 摘 要： | 目的：建立同时测定大鼠血浆中6种CYP450探针药物的方法，并采用Cocktail法研究白香丹胶囊对大鼠CYP450酶活性的影响。方法：选用茶碱(CYP1A2)、氯唑沙宗(CYP2E1)、甲苯磺丁脲(CYP2C6)、右美沙芬(CYP2D2)、奥美拉唑(CYP2D1)、咪达唑仑(CYP3A2)作为探针药物。大鼠随机分为对照组和给药组，给药组每天灌胃白香丹胶囊0.675 g?kg-1，对照组灌胃等量生理盐水，连续给药14天。第15天均灌胃给予6个探针药物，于不同时间点眼眶取血，采用LC-MS/MS法测定各探针药物浓度，计算药动学参数并进行组间t检验。结果：与对照组相比，给药组茶碱(P < 0.01)、氯唑沙宗(P < 0.01)、甲苯磺丁脲(P < 0.05)代谢显著加快，右美沙芬、奥美拉唑、咪达唑仑代谢无显著性差异。结论：白香丹胶囊对大鼠CYP1A2有中强诱导作用，对CYP2E1、CYP2C6有弱诱导作用。
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| 关 键 词： | 白香丹胶囊；CYP450酶；Cocktail法；高效液相色谱串联质谱法 |
| 收稿时间： | 2015-11-06 |
| 修稿时间： | 2016-02-17 |
Influence of Baixiangdan Capsule on Metabolic Activities of Cytochrome P450 Enzymes in Rats by Cocktail Method |
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| SUN Bing-ting,ZHANG Qian,ZONG Yang,HE Shu-fen,ZHU Li-jing and JU Wen-zheng. Influence of Baixiangdan Capsule on Metabolic Activities of Cytochrome P450 Enzymes in Rats by Cocktail Method[J]. Jiangsu Pharmacertical and Clinical Research, 2016, 24(1) |
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| Authors: | SUN Bing-ting ZHANG Qian ZONG Yang HE Shu-fen ZHU Li-jing JU Wen-zheng |
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| Affiliation: | Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Department of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine |
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| Abstract: | |
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| Keywords: | Baixiangdan Capsule Cytochrome P450 enzymes Cocktail method LC-MS/MS |
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