Ischemic and Ozone Oxidative Preconditioning in the Protection Against Hepatic Ischemic-Reperfusion Injury |
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Authors: | Hussam Hassan Ajamieh Silvia Menéndez Nelson Merino Gregorio Martínez-sánchez L Re Olga S León |
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Affiliation: | 1. Center of Research and Biological Evaluations. Food Pharmacy Institute. Havana University.;2. Ozone Research Center. POBox 6412. Havana city. Cuba.;3. Institute of Experimental and Clinical Medicine, Laboratory of Pharmacology, Univ. Ancona, Via Ranieri 2 60131 Ancona, Italy |
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Abstract: | It has been demonstrated that ozone, probably through a mechanism of oxidative preconditioning (OP), protected the liver against the damage mediated by reactive oxygen species. Taking into account that iscehmic preconditioning (IschP) is also a protective mechanism, a comparative study between both preconditioning settings was performed in order to study the effectiveness of both protective procedures. Rats were randomly divided into 4 groups: 1- control, sham operated (anesthesia and laparotomy plus surgical manipulation); 2- I/R (ischemia for 90?min followed by 90?min reperfusion); 3- Ischp+I/R, as group 2 but submitted to a previous ischemic preconditioning (ischemia 10?min and reperfusion 10?min); 4- OzoneOP+I/R, as group 2 but submitted to a pervious oxidative preconditioning with 15 sessions, daily, of ozone by rectal administration (dose of 1?mg/kg). The comparison between both preconditionings showed no biochemical differences for the parameters evaluated. Nevertheless, the histological study demonstrated that the protective effect produced by the OzoneOP is superior to that achieved with the IschP. |
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Keywords: | Ozone Medical Applications Ozone Therapy Liver Ischemia/Reperfusion |
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