血管内皮生长因子与妊娠高血压综合征发病及一氧化氮的关系

目的　探讨血管内皮生长因子（VEGF）在妊娠高血压综合征（妊高征）发病中的作用，及其与一氧化氮（NO）的关系。方法　选择妊高征患者(妊高征组)41例，其中轻度妊高征12例，中度妊高征13例，重度妊高征16例；选择同期正常晚期妊娠妇女20例为对照组。采用酶联免疫吸附法测定两组孕妇血清VEGF水平，用硝酸盐还原酶法测定两组胎盘组织NO浓度变化。结果　（1）妊高征组血清VEGF水平明显低于对照组，轻度妊高征患者血清VEGF水平与对照组比较，差异无显著性，中、重度妊高征患者血清VEGF水平分别为（23.1±4.1）ng/L、（14.8±3.9）ng/L，明显低于对照组。（2）妊高征组胎盘组织中NO浓度较对照组明显降低，轻度妊高征患者胎盘组织NO浓度与对照组比较，差异无显著性，中、重度妊高征患者胎盘组织NO浓度分别为（9.1±2.1）μmol/g、(5.6±1.8)μmol/g，均明显低于对照组。（3）血清VEGF水平与胎盘组织NO浓度呈显著正相关（r=0.65,P<0.01）。结论　妊高征患者血清中VEGF水平降低，胎盘组织中NO浓度下降，可能在妊高征的发病中起一定作用。

Role of vascular endothelial growth factor in the genesis of pregnancy induced hypertension and its relationship with nitric oxide

OBJECTIVE: To study the role of vascular endothelial growth factor in the genesis of pregnancy induced hypertension (PIH) and its relationship with nitric oxide. METHODS: Serum vascular endothelial growth factor(VEGF) levels in 41 women with PIH and 20 normal pregnant women were measured by enzyme linked immunosorbent assay. The concentrations of nitric oxide (NO) in placental tissues were assayed by nitrite reductase. RESULTS: (1) Serum levels of vascular endothelial growth factor VEGF in PIH group were significantly lower than those in normal pregnancy. There was no difference between the mild PIH and normal group in the concentrations of VEGF. VEGF levels in moderate (23.1 +/- 4.1) ng/L and severe PIH (14.8 +/- 3.9) ng/L were significantly lower than those in normal group (P < 0.01). (2) The concentrations of NO in placental tissues in PIH group were significantly lower than those in normal pregnancy (P < 0.01). There was no difference between the concentrations of NO in mild PIH and those in normal pregnancy (P > 0.05). NO levels in moderate PIH (9.1 +/- 2.1) mumol/g and severe PIH (5.6 +/- 1.8) mumol/g were significantly lower than those in control group. (3) The serum levels of VEGF positively correlated with the concentration of NO in placental tissues. (r = 0.65, P < 0.01). CONCLUSION: The serum levels of VEGF and the concentrations of NO in women with PIH are both suppressed. The changes of VEGF levels might play an important role in the pathogenesis of PIH.