手术对肺癌患者外周血中癌细胞端粒酶活性的影响

目的:检测肺癌患者手术前后外周血中癌细胞端粒酶活性,了解手术对其影响及意义。方法:应用端粒酶重复序列扩增法(PCR-TRAP)检测30例肺癌患者手术前及术后第5天外周血中癌细胞端粒酶活性,随访其预后。本院同期30例非肿瘤患者为对照组。结果:非肿瘤患者外周血端粒酶活性均为阴性,肺癌患者手术前后阳性率分别为60%(18/30)及50%(15/30),手术前后阳性率无显著差异(P>0.05),手术后有6例端粒酶活性上升,其中4例存活不足1年;15例端粒酶活性明显下降(P<0.01),其中6例转阴性,15例存活均超过1年。结论:肺癌患者外周血端粒酶活性可能对诊断、指导治疗、判断预后有参考价值,手术治疗能减少外周血中癌细胞。

Effect of operative surgery on telomerase activity in peripheral blood of patients with lung cancer

Objective:To detect the telomerase activity in peripheral blood of patients with lung cancer before and after operative surgery and discuss the influence of operation and its clinical significance.Methods:The peripheral blood was collected from 30 lung cancer patients.The telomerase activity of peripheral blood cancer cells was determined using enzymelinked immunosorbant assay based on the telomeric repeat amplification protocol (ELISA-TRAP) before operation and postoperative day 5 post surgery.All the patients were followed up for 36 months.The control group included 30 patients with non tumor disease.Results:The telomerase activity was negative in the peripheral blood of all the patients with non tumor diseases.For lung cancer patients the positive rate of telomerase activity before and after operation had no difference 60%(18/30) vs 50%(15/30),P>0.05].The telomerase activity was increased in 6 lung cancer patients after operation,among them 4 out of 6 patients did not survive for 1 year.The telomerase activity was decreased in 15 lung cancer patients before and after operation(P<0.01).It changed to negative in 6 out of 15 lung cancer patients.All the 15 patients survived for more than 1 year.Conclusion:Telomerase activity in the peripheral blood of lung cancer patients may have reference value for diagnosis,guide for therapeutics,and determination of prognosis.Operation could reduce the number of cancer cells in peripheral blood.