| 低能N+离子注入谷氨酸产生菌诱变选育及其发酵的研究 |
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| 作者姓名： | 甄卫军 李茜 张石峰 曾宪贤 高林 李江 |
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| 作者单位： | 1. 中国科学院新疆化学所, 乌鲁木齐 830011;2.中国科学院研究生院, 北京 100039;
3. 新疆大学离子束生物工程中心, 乌鲁木齐 830008;
4. 新疆石河子味精厂, 石河子 832000 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金项目(20001);乌鲁木齐重大科技攻关项目(Y0010 )资助 |
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| 摘 要： | 采用低能N+离子注入技术,以D110 和D110B为出发菌进行了诱变选育高产谷氨酸菌株和发酵的试验研究。通过研究已经初筛到两株高产菌株D5221 和B3263,其多批次摇瓶平均产酸分别达到 8.4%、7.5 %,比出发菌分别提高 35.48%和 25 %.其一级种子生长曲线比原出发菌有明显变化,高产菌株发酵对数期平均提前 2～ 3h.从发酵曲线显示出高产菌株的代谢活力增强,倍增时间缩短,对缩短发酵周期有积极的促进作用
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| 关 键 词： | 离子注入 谷氨酸 诱变选育 发酵 |
| 收稿时间： | 2002-01-07 |
| 修稿时间： | 2002-03-05 |
Study of the Mutation Breeding and Fermentation on Glutamic Acid Yielding Bacterium with Low Energy N+Ion Beam Implantation |
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| Authors: | ZHEN Wei Jun LI Qian ZHANG Shi Feng ZENG Xian Xian GAO Lin LI Jiang |
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| Affiliation: | 1. Xinjiang Chemical Institute of the Chinese Academy of Sciences, Urumqi 830011;
2. The Gradudate School of the Chinese Academy of Sciences, Beijing 100039;
3. Center of Ion Beam Bioengineering, Xinjiang University, Urumqi 830008;
4. Xinjiang Shihezi Monosodium Glutamate Plant, Shihezi 832000 |
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| Abstract: | Low energy N+ion implantation technology is used in the mutat ion breeding and fermentation on g lutamic acid yielding start ing st rains of D110 and D110B.It is showed in this study that tw o high yielding glutamic acid st rains D5221 and B3263 are screened out and the average rocker bot tle fermentation GA(%)can achieve 8.4 %、7.5 %, they are respectively raised by 35.48 %、25 % compared to the original strains.There are clearly improved in the aspect on the fi rst class seed fermentation curve.And thei r log arithm phase has generally improved by 2 ～ 3 h.From the fermentation curve it is showed that thei r metabolic capacity are improved, the multiplicat ion cycle are shortened.So it w ill play a positive role in reducing the fermentation cycle. |
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| Keywords: | ion implantation glutamic acid mutation breeding fermentation |
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