| COLNING AND ANALYSIS OF THE GENOMIC DNA SEQUENCE OF AUGMENTER OF LIVER REGENNERATION FROM RAT |
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| 作者姓名： | 董菁 王勤怀 |
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| 作者单位： | [1]GeneTherapyResearchCenter,InstituteofInfectiousDiseases,the302HospitalofPLA,Beijing100039 [2]DepartmentofCommunicableDisease,theFirstHospitalofPekingUniversity,Beijing100034 |
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| 摘 要： | Objiect:To search for genomic DNA sequence of the augmenter of liver regeneration(ALR) of rat.Methods:Polymerase chain reaction(PCR)with sepcific primers was used to amplify the sequence from the rat genome.Results:A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified.The lengths of the gene and pseudogene are 1508 bp and 442 bp,respectivel.The ALR gene of rat includes 3 exons and 2 introns.The 442 bp DNA sequence may represent a pseudogene or a ALR-related petide.Predicted amino acid sequence analysis showed that threre were 14 different amino acid residues between the gene and pseudogene.ALR-related petide is 84 amino acid residues in length and relates closely to ALR protein.Conclusion:There might be a multigene family of ALR in rat.
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| 关 键 词： | 大鼠 肝再生 基因组DNA 克隆序列分析 细胞分裂促进分子 PCR ALR |
CLONING AND ANALYSIS OF THE GENOMIC DNA SEQUENCE OF AUGMENTER OF LIVER REGENERATION FROM RAT |
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| Jing Dong,Jun Cheng,Qinhuan Wang,Shuangshuang Shi,Gang Wang,Chongwen Si.COLNING AND ANALYSIS OF THE GENOMIC DNA SEQUENCE OF AUGMENTER OF LIVER REGENNERATION FROM RAT[J].Chinese Medical Sciences Journal,2002,17(2):63-67. |
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| Authors: | Jing Dong Jun Cheng Qinhuan Wang Shuangshuang Shi Gang Wang Chongwen Si |
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| Affiliation: | Gene Therapy Research Center, Institute of Infectious Diseases, the 302 Hospital of PLA, Beijing 100039. |
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| Abstract: | OBJECTIVE: To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat. METHODS: Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome. RESULTS: A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein. CONCLUSION: There might be a multigene family of ALR in rat. |
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