小鼠巨细胞病毒PCR 诊断方法的建立及其应用

摘要: 目的建立小鼠巨细胞病毒( MCMV) PCR 检测方法，并进行初步应用。方法根据NCBI 公布的巨细胞病毒设计引物，进行PCR 体系优化、敏感性、特异性测试; 运用优化后的方法，对87 份小鼠血清进行检测，对阳性样本进行测序分析。结果建立的小鼠巨细胞病毒PCR 方法灵敏度高，特异性好，初步检测4 个屏障设施的87 份小鼠血清， 13 份为阳性，通过测序证实为小鼠巨细胞病毒，验证了此方法的可行性。结论为小鼠巨细胞病毒的快速检测提供了方法。

Establishment and Application of a PCR Method for Detection of Murine Cytomegalovirus

Abstract: To establish a PCR assay for detection of murine cytomegalovirus ( MCMV) ． Provide help for the purification of MCMV． Sequence of MCMV was obtained from NCBI Genbank． One pairs of primers were designed based on this sequence． The primers was determined through amplifying the major strains in China． Eighty-seven mice serum were tested with this PCR assay． The positive amplified fragments were sequenced to verify the reliability of this method． A PCR method for detection of MCMV is successfully established，with a high specificity and sensitivity． Among the tested 87 blood samples， 13 positive cases were detected． Sequencing of the samples are consistent with the amplification results，and verified the applicability of this detection method． The establishment of this method provides the basis for detection of MCMV in mice，and application of this assay in mouse．