我国狂犬病疫苗生产用毒株糖蛋白氨基酸序列及其抗原位点分析

目的分析我国狂犬病疫苗生产用毒株aG(PG)株和CTN株糖蛋白结构及抗原位点的差异。方法登录GenBank搜寻aG株和CTN株糖蛋白的氨基酸序列,用Vector NTI Suite8软件分析两毒株糖蛋白的同源性;用DNAStar5分析两毒株糖蛋白的二级结构和潜在的抗原位点。结果aG株和CTN株的糖蛋白氨基酸序列的同源性为88·2%,特征性二级结构的位置与数量相似,主要的抗原位点均为13个。结论两毒株的糖蛋白氨基酸序列虽然存在一定的差异,但生产的狂犬病疫苗均能产生较好的保护效果,提示某些表位是产生中和抗体的关键。

Analysis of Amino Acid Sequences and Antigen Epitopes of Glycoprotein of Ra-bies Vaccine Virus Strains in China

Objective To compare the structures and antigen epitopes of glycoprotein of aG(PG) and CTN strains used for production of rabies vaccine in China.Methods Search the amino acid sequences of glycoprotein of aG and CTN strains in GenBank and compare the homology of the two strains by Vector NTI Suite 8 software.Analyze the secondary structures and potential antigen epitopes of the two strains by DNAStar 5 software.Results The homology of amino acid sequences of glycoprotein of aG and CTN strains were 88.2%,and the sites and numbers of their characteristic secondary structures were basically consistent.Both the glycoprotein contained thirteen epitopes.Conclusion The amino acid sequences of glycoprotein of aG and CTN strains showed a certain difference.However,both the vaccines prepared with the two strains showed good protective effect.It indicated that some antigen epitopes played key roles in the induction of neutralizing antibody.