肝素抗凝血浆和血清的肝纤维化标志物检测结果比较

目的对比观察血浆和血清中Ⅳ型胶原(CIV)、透明质酸(HA)、Ⅲ型前胶原(PCⅢ)和层黏蛋白(LN)的测定结果。方法采用放射免疫法对56例健康人的血清与肝素抗凝血浆进行肝纤维化4项标志物测定,并对检测结果进行比较。结果血清中CIV、PCⅢ、LN含量大于血浆中的含量,差异有统计学意义(P0.05);血浆中HA含量则大于血清中的含量,差异也有统计学意义(P0.05)。血浆CIV、HA、PCⅢ含量与血清含量存在相关关系(P0.001);而LN含量不存在相关关系,差异无统计学意义(P0.05)。结论血浆可测定肝纤维化4项标志物,且3项标志物与血清存在相关关系。由于其CIV、PCⅢ、LN含量小于血清含量,因此,临床仍采用血清测定肝纤维化4项标志物,但应重视血清HA含量低于正常值范围的肝纤维化待查者。

Comparison of the test results of heparinized plasma and serum hepatic fibrosis markers

Objective To observe the test results of plasma and serum collagen type IV（CIV）,hyaluronic acid（HA）,procollagen typeIII（PCIII）and laminin（LN）.Methods The 4 hepatic fibrosis markers in the serum and heparinized plasma samples of 56 healthy subjects were measured with radio-immunoassay,and the results were compared.Results The contents of CIV,PCIII and LN in serum were higher than those in plasma（P〈0.05）,while the contents of HA in plasma were higher than those in serum（P〈0.05）.The contents of CIV,HA and PCIII in plasma were correlated with those in serum（P〈0.001）.However,the correlation was not observed in the contents of LN（P 0.05）.Conclusion The 4 hepatic fibrosis markers can be measured in plasma,in which there are 3 markers correlated with the same ones in serum.Since the contents of CIV,PCIII and LN in plasma were lower than those in serum,and the 4 hepatic fibrosis markers must still be measured in serum for clinical practice.Great attention must be paid to the persons with serum HA contents lower than normal value ranges when they want to be examined for hepatic fibrosis.