T淋巴细胞在小鼠肾缺血再灌注损伤中的作用

目的研究T淋巴细胞在肾缺血再灌注损伤(IRI)导致的急性肾损害中的作用。方法BALB/c小鼠和BALB/c裸小鼠各24只,分别随机分为A1-4组和B1-4组,每组6只。双肾蒂阻断45 min后恢复血流建立肾IRI模型,假手术对照组I、RI后24、48和72 h时检测Scr、尿蛋白定量及肾病理学,A组检测脾T细胞亚群;对比BALB/c小鼠和BALB/c裸小鼠的肾功能下降、组织学损害程度以及脾T淋巴细胞亚群变化。结果A2-4组和B2-4组均有Scr和尿蛋白定量明显升高(P<0.05),且A组损害程度明显重于B组(P<0.05);A2-4组出现典型的IRI组织损害表现(P<0.05),B2-4组无明显IRI组织损害(P>0.05);A2-3组脾CD3 T细胞百分比较A1组升高(P<0.05),而CD4 /CD8 比值无明显变化(P>0.05)。结论T淋巴细胞是小鼠肾IRI导致急性肾损害的重要病理生理学因素。

Pathophysiologic Role of T Lymphocytes in Renal Ischemia-Reperfusion Injury in Mice

Objective To investigate the pathophysiologic role of T lymphocytes in renal ischemia-reperfusion injury in mice.Methods BALB/c wild-mice(group A, n = 24) and BALB/c nude-mice(group B,n = 24) were divided into four groups(n = 6),respectively,the groups A-1 to A-4 and groups B-1 to B-4.Bilateral renal pedicles were occluded by microvascular clamps for 45 minutes.Then the kidneys were reperfused.Samples were collected at sham operation and 24 h,48 h and 72 h after IRI.Serum creatinine(Scr),urine protein concentration,renal histology and splenic CD3~ /CD4~ /CD8~ positive cells(group A) were examined,respectively.Results\ Scr and urine protein concentration increased and histological injury was observed in group A-(2-4) and B-(2-4).The injury in group A were more severe than that in group B(P<0.05).FACS analysis showed,that in comparison with group A-1,percentage of splenic CD3~ T cells in group A-2 and A-3 increased significantly(P<0.05),but a similar ratio of CD4~ /CD8~ between group A1 and group A-(2-3)(P>0.05)).Conclusion T lymphocytes play an important pathophysiologic role in renal ischemia-reperfusion injury,and may mediate post-ischemic decline in renal function and lead to histological injury.