前列腺癌细胞凋亡与iNOS、Bcl-2的表达及意义

目的 :探讨细胞凋亡和诱生型一氧化氮合酶 (iNOS)、Bcl 2蛋白表达在前列腺癌中的意义以及两者之间的关系。　方法 :对 2 4例前列腺癌、15例前列腺增生及 5例正常前列腺组织行原位细胞凋亡检测及iNOS、Bcl 2蛋白免疫组化检测。　结果 :前列腺癌组的细胞凋亡率和iNOS的染色强度均显著高于前列腺增生组及正常前列腺组(P <0 .0 1) ,且Bcl 2蛋白表达阳性者的细胞凋亡率显著低于阴性者 (P <0 .0 1)。　结论 :前列腺癌的细胞凋亡率可反映其恶性程度 ;iNOS的表达与前列腺癌的分化程度无关 ,但与Bcl 2蛋白表达呈负相关 ;Bcl 2蛋白表达与前列腺癌细胞凋亡呈负相关。iNOS与Bcl 2均可通过影响前列腺癌细胞凋亡而在前列腺癌病理发生发展中起作用

The Apoptosis and Its Relationship with the Expression of Inducible Nitric Oxide Synthase and Bcl-2 Proteins in Prostate Carcinoma

Objective: To study the relationship between the apoptosis and the expression of inducible nitric oxide synthase(iNOS) and Bcl-2 in prostate carcinoma (PCa). Methods: Expression of Bcl-2 and iNOS and apoptotic cells in 24 cases of PCa, 15 cases of BPH and 5 cases of normal prostate tissues were detected by immunohistochemical technique and terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL), respectively. Results: Apoptosis index (AI) and iNOS-positive index of PCa were much higher than that of the benign prostate hyperplasia (BPH) and the normal prostate group(P< 0.01). The AI of the Bcl-2-positive group was lower than that of the negative ones in PCa(P< 0.01). Conclusions: AI might serve as a marker in evaluating the aggression of PCa. The iNOS-positive index of PCa had no relationship with the differentiated grades but had a negative relationship with the expression of Bcl-2. The expression of Bcl-2 protein was negatively related to PCa cell apoptosis. Both iNOS and Bcl-2 were believed to play roles in the pathogenesis and development of PCa by influencing the cell apoptosis.