泥鳅多糖对小鼠脾细胞免疫应答的影响

目的:研究泥鳅多糖对小鼠脾细胞免疫应答的影响.方法:分别给正常小鼠、刀豆蛋白(ConA)或左旋咪唑免疫增强小鼠、环磷酰胺免疫抑制小鼠腹腔注射提取的泥鳅多糖,连续给药7d.用~H-胸苷掺入法测定T淋巴细胞的增殖.用~(51)Cr同位素标记法测定细胞毒T淋巴细胞的细胞毒性和天然杀伤细胞的活性.结果:泥鳅多糖5或10mg·kg~(-1)·d~(-1)可以提高T淋巴细胞的增殖,并增强细胞毒T淋巴细胞的细胞毒性和天然杀伤细胞的活性,还能增强ConA解除环磷酰胺对T淋巴细胞增殖抑制的能力,抑制率从环磷酰胺对照组的51.4％分别降低到18.2％和35.1％.而且,给予小鼠10或20mg·kg~(-1)·d~(-1)的泥鳅多糖,可以恢复环磷酰胺所致的天然杀伤细胞活性降低.结论:泥鳅多糖能增强小鼠脾细胞中T细胞、细胞毒T淋巴细胞和天然杀伤细胞的活性.

Effect of Misgurnus anguillicaudatus polysaccharide on immune responses of splenocytes in mice

AIM: To investigate the effect of Misgurnus anguillicaudatus polysaccharides (MAP) on immune responses of splenocytes in mice. METHODS : T lymphocyte proliferation (TLP) was measured by 3H]thymidine incorporation assay. Cytotoxic T lymphocyte (CTL) cytotoxicity and natural killer (NK) activity were determined by release of radioactive chromium 51Cr] from pre-labeled target cells. RESULTS: MAP 5 and 10 mg . kg-1 . d-1, ip for 7 d, could increase TLP, enhance the cytotoxicity of CTL and NK cells, and antagonize the effect of concanavalin A (ConA) on TLP suppressed by cyclophosphamide (CP). Inhibitory rates of CTL cytotoxicity were decreased from 51.4 % in CP control mice to 18.2 % and 35.1 % in MAP-treated CP mice, respectively. Furthermore, administration of MAP 10 and 20 mg . kg-1 . d-1, ip for 7 d, restored the reduced NK cell cytotoxicity caused by CP administration in mice. CONCLUSION: MAP has protective effect on augmenting T-cell-mediated immunity and NK activity in normal and CP-treated mice.