结构蛋白及血管形成因子在体表海绵状静脉畸形中的表达及意义

背景体表海绵状静脉畸形,又称为海绵状血管瘤,是一种较常见而难治的血管疾病,目前对其发病机制了解甚少.目的研究结构蛋白及血管形成因子在体表海绵状静脉畸形 (cavernous venous malformation,CVM)中的表达及意义.设计研究 CVM病理标本中的结构蛋白和血管形成因子的表达,用正常中型静脉和小静脉标本作为对照,半定量分析结果.地点和对象解放军第二军医大学附属长海医院 1996/2000间手术切除的 CVM病理标本 25例,正常中型静脉和小静脉标本各 12例.干预采用 Envision法免疫组化染色观察 CVM、正常中型静脉和小静脉标本中的Ⅳ-型胶原、纤维连接蛋白(Fn)、层粘连蛋白(Ln)等结构蛋白及血管内皮细胞生长因子(VEGF)、血管生成素-1(Ang-1)等血管形成因子的表达.主要观察指标观察Ⅳ-型胶原、 Fn、 Ln、 VEGF、 Ang-1在各个标本中的表达强度,分为"-、+、" 3个等级.结果Ⅳ型胶原、 Fn和 Ln在海绵状静脉畸形与在中小静脉中的分布类似,但表达量明显少.畸形组织和小静脉的 VEGF表达明显强于中型静脉,小静脉的 Ang-1表达明显强于静脉畸形和中型静脉.结论结构蛋白及血管形成因子表达变化可能是海绵状静脉畸形形成发展的重要因素.Ang-1表达减少可能参与海绵状静脉畸形的血管塑型障碍的发生.

Expression and significance of structural proteins and vascular growth factors in cavernous venous malformation on body surface

BACKGROUND:Cavernous venous malformation( CVM), also called cavernous hemangioma,is a common kind of vascular disease and hard to cure.At present we know little about the pathogenesis of it. OBJECTIVE:To study the expression and significance of structural proteins and vascular growth factors in cavernous venous malformation of body surface. DESIGN:The expression of structural proteins and vascular growth factors in the CVM specimens was studied.The normal medium-sized veins and small veins were used as the control. A semi-quantitative analysis was used to analyze the result. SETTING and PARTICIPANTS:Twenty-five specimens of cavernous venous malformation,12 specimens of medium-sized veins and 12 specimens of small veins were abtained from Changhai Hospital affiliated to the Second Military Medical University during the year of 1996 to 2000. INTERVENTION:Envision method was used to observe the expression of Ⅳ-collagen,fibronectin(Fn),Laminin(Ln), vascular endothelial growth factor(VEGF) and angiogenin-1(Ang-1) in the above-mentioned specimens. RESULTS: The distribution of Ⅳ-collagen,Fn and Ln in specimens of CVM is similar to that in specimens of moderate and small veins, but the expression of Ⅳ-collagen,Fn and Ln in specimens of CVM is less obviously. The expression of VEGF in specimens of malformations and small veins was significantly higher than that in medium-sized veins. The expression of Ang-1 in specimens of small veins was significantly higher than that in specimens of venous malformation and medium-sized veins. CONCLUSION:The changes of the expression of structural proteins and vascular growth factors may be one of the important factors in the formation and development of CVM. The decreased expression of Ang-1 may be related to the disturbance of vascular remodeling in CVM.