酸枣仁油中脂溶性成分的研究

目的研究酸枣仁的脂溶性化学成分。方法采用索氏提取法提取酸枣仁中的酸枣仁油,测定不皂化物用GB/T 5535.1-2008方法处理,测定脂肪酸用甲酯化方法处理,最后用气相色谱-质谱联用仪测定不皂化物和脂肪酸。结果检出9种不皂化物和6种脂肪酸,其中不皂化物分别为叶绿醇、亚油酸乙酯、油酸乙酯、香叶基醇、角鲨烯、菜油甾醇、豆甾醇、γ-谷甾醇、桦木酸甲酯,其相对含量最多的是γ-谷甾醇和叶绿醇;脂肪酸分别为棕榈酸、松油苯二甲酸、硬脂酸、油酸、十八碳二烯酸、花生油酸,其中油酸和十八碳烯酸的含量最高。结论该方法快速、准确、简便,为进一步研究酸枣仁药用价值提供依据。

Analysis of liposoluble components from the oil of Semen Ziziphi Spinose

Objective To Study the fat-soluble chemical composition of Semen Ziziphi Spinose. Methods The oil of Semen Ziziphi Spinose in samples were extracted by Soxhlet extraction method. The GB/T 5535.1-2008 method was applied to detect the unsaponifiable matter and methyl ester method processing was used to determine fatty acid. The contents of fatty acid and unsaponifiable matter were detected by gas chromatography-mass spectrometry (GC-MS). Results Totally 9 kinds of unsaponifiable matter and 6 kinds of fatty acid was detected in the samples, including unsaponifiable material such as phytol, linoleic acid ethyl ester, ethyl oleate, geranyl alcohol, squalene, campesterol, stigmasterol, gamma sitosterol, and birch acid methyl ester, with the most two of gamma sitosterol and phytol. Fatty acid included palmitic acid phthalate, pine oil, stearic acid, oleic acid, 18 carbon diene acid, arachidic acid, and oleic acid. And the content of 18 carbon olefine and oleic acid were of the highest. Conclusion The method is rapid, accurate, and convenient, and it provides the basis for further study on medicinal value of Semen Ziziphi Spinose.