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| 大孔吸附树脂纯化芦笋皂苷工艺优化 | |
| 引用本文： | 赵芳春,赵兵,黄云祥.大孔吸附树脂纯化芦笋皂苷工艺优化[J].食品工业科技,2012,33(18):248-252. |
| 作者姓名： | 赵芳春 赵兵 黄云祥 |
| 作者单位： | 1. 中国科学院过程工程研究所生化工程国家重点实验室,北京100190 中国科学院研究生院,北京100049 2. 中国科学院过程工程研究所生化工程国家重点实验室,北京,100190 3. 秦皇岛长胜农业科技发展有限公司,河北秦皇岛,066000 |
| 基金项目： | 科技部农业科技成果转化资金项目(2010A20007) |
| 摘 要： | 以芦笋下脚料提取物作为原料,优化了大孔吸附树脂纯化芦笋皂苷的工艺。通过静态法,对8种大孔吸附树脂纯化芦笋皂苷的效果进行比较,结果表明HPD-100树脂纯化芦笋皂苷效果最佳,并对HPD-100大孔树脂纯化芦笋皂苷工艺进行了优化。在上样质量浓度15mg/mL、溶液处理量150mL、1mL/min流速过柱;吸附后,先用50mL蒸馏水洗去杂质,再用200mL80%乙醇洗脱,总皂苷的含量由14.26%提高到35.49%(mean±SD)。将初次纯化产物按照上述工艺再纯化一次,总皂苷含量可高于50%,达到国家五类新药的含量要求。HPD-100可较好地吸附分离芦笋皂苷,纯化效率高,且其操作简单、安全、成本低廉,有较高的推广应用价值。 |
| 关 键 词： | 芦笋 皂苷 大孔吸附树脂 纯化 HPD-100 |
Purification of saponins from Asparagus officinalis by macroporous adsorption resin |
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| ZHAO Fang-chun,ZHAO Bing,HUANG Yun-xiang.Purification of saponins from Asparagus officinalis by macroporous adsorption resin[J].Science and Technology of Food Industry,2012,33(18):248-252. | |
| Authors: | ZHAO Fang-chun ZHAO Bing HUANG Yun-xiang |
| Affiliation: | 1.State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.Qinhuangdao Chang Sheng Agricultural Science & Technology Development Co.,Ltd.,Qinhuangdao 066000,China) |
| Abstract: | An optimal macroporous adsorption resin and its optimal technological parameters for purification of saponins from extracts of Asparagus officinalis were investigated in the present study.Through static method,comparing the saponin purification result of 8 resins,HPD-100 resin was chosen due to its excellent adsorption and desorption performance.Optimal conditions were primarily confirmed as follows:sample concentration 15mg/mL with loading amount of 150mL at a flow rate of 1mL/min,washing with 200mL 80% ethanol at a flow rate of 1mL/min.Sample was purified by HPD-100 resin according to these technology parameters and the content of saponins reached 35.49%.Then the primary purification product was purified in accordance with the above process,and the content of saponins could be higher than 50% and complied with the standards of class V of new Chinese drug.These datas demonstrated the excellence of HPD-100 resin in purifying saponins from A.officinalis and the advantages of the developed method for industrial application,like simple operation,safety and low cost. |
| Keywords: | Asparagus officinalis saponins macroporous adsorption resin purification HPD-100 |
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