| 烟草叶绿体转化载体的构建及转基因植株的获得 |
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| 引用本文： | 邹竹荣,张中林.烟草叶绿体转化载体的构建及转基因植株的获得[J].作物学报,1998,24(4):410-415. |
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| 作者姓名： | 邹竹荣 张中林 |
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| 作者单位： | 中国农业科学院生物技术研究中心，北京，100081 |
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| 基金项目： | 国家自然科学基金，农业部95农-18-03项目 |
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| 摘 要： | 选择烟草叶绿体基因组同源片段ｒｐ１２－ｔｒｎＨ－ｐｓｈｂＡ和ｔｒｎＫ－ＯＲＦ５０９Ａ，及ａａｄＡ抗壮观霉素基因，构建烟草叶绿体转化载体ｐＴＲＺ，制备ｐＴＲＺＤＮＡ金粉子弹，通过基因枪轰击烟草幼苗叶片，经壮观霉素筛选获得了愈伤组织和转化再生植株，对烟草叶绿体转化植株进行ＰＣＲ和Ｓｏｕｔｈｅｒｎ分析，结果证明其中Ｎｏ１３，１６，２３为整入了外源ａａｄＡ的叶绿体转基植株，同时其子代呈现壮观霉素抗性ａａＤ
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| 关 键 词： | 烟草 叶绿体转化 载体 构建 转基因植株 |
| 收稿时间： | 1996-12-13 |
| 修稿时间： | 1997-10-08 |
Construction of Vector for Tobacco Plastid Transformation and Obtainment of Tobacco Transgenic Plants |
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| Zou Zhurong Zhang Zhonglin Shan Song Ni Pichong Shen Guifang.Construction of Vector for Tobacco Plastid Transformation and Obtainment of Tobacco Transgenic Plants[J].Acta Agronomica Sinica,1998,24(4):410-415. |
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| Authors: | Zou Zhurong Zhang Zhonglin Shan Song Ni Pichong Shen Guifang |
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| Affiliation: | Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing,100081 |
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| Abstract: | Vector pTRZ was constructed with rpl2-trnH-psbA, trnK-ORF509A as to
bacco plastid homologous recombinant fragments and spectinomycin-resistant aadA gene from
plasmid pZS197. After spectinomycin selection, transformed calli and regenerated tobacco plants
were obtained from tobacco seedling's leaves bombarded with golden microprojectiles coated with
pTRZ DNA. Further PCR and Southern analyses of transformed plants indicated that No. 13,
16, 23 were positive tobacco transplastomic plants and the foreign aadA gene had integrated into
their plastid genome. The resistance of progeny phenotype to spectinomycin showed the aadA
gene was stably inherited to their progeny. |
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| Keywords: | Plastid homologous recombinant fragment Plastid transformation aadA Microprojectiles bombardment |
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