| FITC—PSA荧光标记法在检测人精子顶体结构中的应用 |
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| 引用本文： | 王晓平,李志斌,李兰.FITC—PSA荧光标记法在检测人精子顶体结构中的应用[J].广西医学,2000,22(5):952-954. |
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| 作者姓名： | 王晓平 李志斌 李兰 |
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| 作者单位： | 广西医科大学第三附属医院泌尿外科 530031 |
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| 摘 要： | 目的：探讨ＦＩＴＣ－ＰＳＡ荧光标记法在检测人精子顶体结构中的临床应用价值。方法：应用络合异硫氰酸荧光素的碗豆凝集素（ＦＩＴＣ－ＰＳＡ）进行精子顶体荧光标记，分别测定５８例不育患者及４６例正常生育男子的精液标本，比较两组间精子顶体完整率的差异。结果：正常生育组的精子顶体完整率显著高于不育组（Ｐ〈０．０１）。结论：ＦＩＴＣ－ＴＳＡ荧光标记法操作简单，技术结果可靠，特异性强，可作为评价人精子授精能力的一
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| 关 键 词： | 精子 顶体结构 检测 FITC－PSA荧光标记法 |
Application of fluorescein-labeled pisum sativum agglutinin to detection of structure of human sperm acrosome |
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| Wang Xiaoping,Li zhibing,Li Lan.Application of fluorescein-labeled pisum sativum agglutinin to detection of structure of human sperm acrosome[J].Guangxi Medical Journal,2000,22(5):952-954. |
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| Authors: | Wang Xiaoping Li zhibing Li Lan |
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| Affiliation: | Nanning 530031 |
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| Abstract: | Objective: To evaluate the clinical application of fluorescein-labeled pisum sativum agglutinin to detection of structure of human sperm acrosome Methods:The rate of acrosome-intact was detected using Fluorescein-labeled pisum sativum agglutinin,and the difference between fertile men and infertile men were compared Result:the acrosome-intact rate in fertile men was higher signilicantly than that in infertile men(P<0 01) condusions:The authors consider that the simple and reliable PSA fluorescent staining is a valuable method for detection of human sperm acrosome and assessment of the fertilizing ability |
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| Keywords: | Sperm Acrosome Pisum sativum agglutinin |
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