仙人掌果多糖的药效研究

目的:探讨仙人掌果多糖提取物(CPFP)的降血糖、降血脂、降血压及抗肿瘤作用。方法:以链脲佐菌素(STZ)诱导SD大鼠成糖尿病模型,随机分为模型组、胰岛素组(10 U.kg-1.d-1,sc给药)、CPFP高、低剂量组(2.37,1.58 g.kg-1.d-1),连续ig 8周,观察各组大鼠血糖的变化;以高脂饲料饲养SD大鼠3周,建立高脂血症模型后,随机分为模型组,洛伐他汀组(3.0 mg.kg-1.d-1)、CPFP高、低剂量组(2.37,1.19 g.kg-1.d-1),连续ig 3周,观察各组大鼠血脂的变化;15周自发性高血压大鼠(SHR)50只,随机分为模型组、卡托普利组(0.01 g.kg-1.d-1)、CPFP高、中、低剂量组(2.37 g.kg-1.d-1,1.58,0.76 g.kg-1.d-1),同龄正常血压的Wistar大鼠10只为正常对照组,连续ig 9周,每周测血压1次,观察各组大鼠血压的变化;建立S180荷瘤小鼠移植瘤模型,随机分为模型组、环磷酰胺组(CTX,0.02 g.kg-1.d-1,ip给药)、CPFP高、中、低剂量组(1.97,0.99,0.49 g.kg-1.d-1),连续ig 10 d,检测CPFP对荷瘤小鼠的抑瘤率。结果:与模型组比较,CPFP可明显降低糖尿病大鼠血糖(P<0.05或P<0.01),CPFP高、低剂量组的降糖率分别为54.08%,44.73%;与模型组比较,CPFP给药组血清总胆固醇(TC)、甘油三脂(TG)和低密度脂蛋白-胆固醇(LDL-C)水平显著降低(P<0.01),CPFP高剂量组高密度脂蛋白-胆固醇(HDL-C)水平明显升高(P<0.05);9周后各给药组大鼠的血压较SHR模型组明显降低(P<0.01),而模型组血压明显高于正常组大鼠(P<0.01);给药10 d后,CPFP高、中剂量组对肿瘤均有明显的抑制率,抑瘤率分别为49.70%,31.13%。结论:CPFP具有明显降低STZ诱导的糖尿病大鼠血糖、降低高血脂症大鼠血脂、降低SHR血压及抗肿瘤作用。

Pharmacodynamic Study on Cactus Fruit Polysaccharide Extract

Objective: To explore antihyperglycemic, antihyperlipidemic, antihypertensive and anti-tumor effects of the cactus fruit polysaccharide extract(CPFP). Method: The diabetic rats were induced by STZ in SD rats, and randomly divided into model group, insulin group(10 U·kg^-1·d^-1), high-dose group of CPFP(2.37 g·kg^-1·d^-1), low-dose group of CPFP(1.58 g·kg^-1·d^-1). The CPFP were administrated daily via gavage for eight weeks. During the experiment, the blood glucose of rats were determined. Male SD rats were fed by hyper-fatty diet for 3 weeks in order to establish the hyperlipemia rat model. The hyperlipemia rats were randomly divided into model group, lovastatin group(3.0 mg·kg^-1·d^-1), high-dose group of CPFP(2.37 g·kg^-1·d^-1), low-dose group of CPFP(1.19 g·kg^-1·d^-1). Different dose of CPFP were administrated daily via gavage for 3 weeks. The blood lipoprotein of rats were determined. Fifty male spontaneous hypertension rats(SHR)at the age of 15 weeks were randomly divided into model group, captopril group(0.01 g·kg^-1·d^-1), high-dose group of CPFP(2.37 g·kg^-1·d^-1), middle-dose group of CPFP(1.58 g·kg^-1·d^-1), low-dose group of CPFP(0.76 g·kg^-1·d^-1). Ten male Wistar rats were served as normal group. Different dose of CPFP were administrated daily via gavage for 9 weeks. The systolic blood pressure(SBP)of experimental rats was detected weekly. S180-tumor bearing mice were established and divided into model group, cyclophosphamide(CTX)group(0.02 g·kg^-1·d^-1), high-dose group of CPFP(1.97 g·kg^-1·d^-1), middle-dose group of CPFP(0.99 g·kg^-1·d^-1), low-dose group of CPFP(0.49 g·kg^-1·d^-1). The CPFP were administrated daily via gavage for 10 days. Tumor inhibition rates were recorded. Result: Compare with model group, the content of blood glucose was remarkably decreased and the hypoglycemic rates were 54.08% and 44.73% respectively in high-dose and low-dose group of CPFP. The levels of TC, TG and LDL-C were decreased significantly(P<0.01)in high-dose and low-dose group of CPFP. The level of HDL-C was obviously increased in high dose group of CPFP(P<0.05). The SBP level of SHR in CPFP treatment groups was depressed notability, nevertheless the SBP level of SHR of model group was increased obviously comparing with normal group after 9 weeks. The tumor was inhibited in CPFP treatment groups. The tumor inhibited rates were 49.70%, 31.13 in high-dose, middle-dose group of CPFP respectively after 10 days. Conclusion: CPFP could markedly decrease blood glucose in STZ-induced diabetic rats and decrease blood lipoprotein in hyperlipemia rats. CPFP showed effects on reducing blood pressure in SHR and certain anti-tumor.