甘蓝型油菜花瓣缺失基因的图谱定位

在无花瓣品系APT02和正常有花瓣品种中双4号构建的的F2分离群体中，运用AFLP和SRAP两种标记技术对甘蓝型油菜花瓣缺失基因进行分子标记和图谱定位。在两亲本间筛选20对AFLP引物和170对SRAP 引物，进一步通过BSA法筛选，获得了与甘蓝型油菜花瓣缺失基因WHB连锁的1个SRAP标记e8m3_4（600bp）和1个AFLP标记E3247_15（150bp），标记与基因WHB之间的遗传距离分别为5 cM和13.5cM；构建了一个甘蓝型油菜(Brassica napus.L )的分子标记遗传连锁图谱，该图谱共包含213个AFLP标记、56个SRAP标记和1个形态标记，分布于17个主要连锁群、两个三联体和4个连锁对中，遗传图距总长2487.1cM，标记间平均距离为10.09 cM。通过图谱定位，控制花瓣缺失性状的基因WHB被定位到第4连锁群(LG4)上。

Molecular mapping of the gene(s) controlling petal-loss trait in Brassica napus

An F_2 population derived from a cross between apetalous line 'APT02'and normal petalled cultivar 'ZS No.4' was used for molecular marker searching and chromosomal mapping of the gene(s) controlling petal-loss trait in Brassica napus.Twenty pairs of AFLP primers and 170 pairs of SRAP primers were selected and screened from two parents.In further selection through bulked segregant analysis(BSA)approach,one SRAP marker e8m3_4(600 bp) and one AFLP marker E3247_15(150 bp)were obtained and found to be linked to the gene(s) controlling petal-loss trait,with the genetic distance of 5 cM and 13.5 cM.A linkage map in Brassica napus was constructed.It consisted of 213 AFLP,56 SSR loci and a morphology marker throughout 17 main linkage groups,two triplet and four linkage pairs.Total length of the map covered 2 487.1 cM,and average interval between markers was 10.09 cM.By genetic mapping,the gene(s) controlling petal-loss trait(WHB)was mapped in LG4.