Effects of ferrous iron and transferrin on cell proliferation of human diploid fibroblasts in serum-free culture |
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Authors: | Mikio Kan Isao Yamane |
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Affiliation: | (1) Cell Biology Division, Research Institute for Tuberculosis and Cancer, Tohoku University, 980 Sendai, Japan |
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Abstract: | Summary Iron-free RITC 80-7 defined medium was used to examine effects of ferrous iron and transferrin on cell proliferation of human
diploid fibroblasts. Both ferrous iron and holotransferrin stimulated cell proliferation in the medium, but apotransferrin
did not. When 5 g/l human serum albumin (HSA) was added to the defined medium, excellent growth was obtained under hypoxic
conditions, whereas a reduction of cellular growth during the culture periods was observed under aerobic conditions. When
ferrous iron was added to the HSA medium alone, the reduction in growth increased in proportion to the concentrations, whereas
the addition of transferrin prevented this reduction in a concentration-dependent manner. This suggests that the ferrous iron
concentration in media causes a reduction in growth under aerobic conditions and transferrin prevents this reduction because
it decreases the ferrous iron concentration. Further, serum albumin seems to be a source of iron in media. |
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Keywords: | ferrous iron human diploid fibroblasts human serum albumin oxygen-dependent reduction in growth transferrin |
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