hNIS基因转染介导碘-131杀伤肝癌细胞的研究

目的 将hNIS基因转染到肝癌细胞使其具备主动吸收碘的功能,观察转基因肝癌细胞吸收碘的能力,并进一步观察其介导放射性碘杀伤肝癌细胞的可能性。方法 重组含有hNIS的载体,并转染至肝癌细胞HepG2,利用稳定表达的转基因肝癌细胞进行吸碘能力测定,并应用MTT法测定碘-131杀伤肝癌细胞的效果。结果 γ计数仪测定表达hNIS基因的肝癌细胞吸收碘的能力比对照组高10倍,MTT法测定结果显示表达hNIS基因的肝癌细胞抑制率明显高于对照组。结论 转基因肝癌细胞具有浓聚碘的能力,并且可以介导碘-131杀伤肝癌细胞。

Research of Kill the Liver-cancer Cells by Transfect hNIS Gene into It Acrosse Radioactive Iodine Uptake

Objective We transfected the human sodium / iodide symporter（hNIS） gene into liver-cancer cells to help it have the ability of iodine uptake to assess the effect of radioactive iodine uptake by liver-cancer cells and the role of anti-hepatoma by radioactive iodine.Methods Recombinant pCDNA-hNIS was transfected into HepG2 cells.The level of 131Ⅰuptake was assessed using transgenic liver cells stably expressing hNIS and effect of anti-hepatoma was determined by MTT method.Results 10-fold 131Ⅰuptake was observed in HepG2 cells transfected with pCDNA-hNIS compared with the control group by γ counting,correspondingly.The effect of anti-hepatoma was significantly higher in transgenic HepG2 cells compared with the controls.Conclusion HepG2 cells transfected with pCDNA-hNIS could uptake 131Ⅰ,accordingly,inhibited by 131Ⅰ.