不同炮制方法对紫金砂中成分及活性的影响

目的 研究不同炮制方法对紫金砂成分及其体外抑制胃H+/K+ -ATP酶活性的影响.方法 采用HPLC法测定紫金砂醇提物中没药烷吉酮,并利用H +/K+ -ATPase试剂盒测定紫金砂醇提取物对离体培养小鼠胃壁细胞中H+/K+-ATP酶活性的影响.结果 紫金砂不同炮制品中没药烷吉酮量有较大变化,其中麸烘样品没药烷吉酮量下降最严重,减少达50％;各炮制品醇提物与阴干样品的成分进行指纹图谱比对时,存在峰缺失和含有量变化.阴干、酒蒸、炒黄、麸炒、麸烘、醋炒、土炒、姜汁炙、米泔水制9种炮制品醇提物对H+/K+-ATP酶的IC50按生药量计算依次为144.93、123.28、283.04、148.44、375.38、129.86、171.50、76.64和120.70 μg/mL.结论 紫金砂不同炮制品所含成分和H+/K+-ATP酶抑制活性均存在明显的差异,其中,姜汁炙抑制活性最好,推荐使用姜汁炙炮制紫金砂饮片.

Effect of processing on components and activity of Angelica polymorpha

AIM To study the impact of different processings on the components of Angelica polymorpha and its inhibitory effect on gastric H+/K+-ATPase activity.METHODS Bisabolangelone content in ethanolic extracts of Angelica polymorpha was determined by HPLC.The H+/K+-ATPase inhibitory activity on the mouse’s gastric parietal cells in vitro was measured by H+/K+-ATPase activity determination kit,which was used to the influence on the ethanolic extracts of different processings in Angelica polymorpha.RESULTS Bisabolangelone content in processings decreased,especially up to 50% in the sample processed with stir-frying with bran.And the changes in peak number and contents were found out as compared with the sample of drying in shade.IC50 of H+/K+-ATPase were obtained from ethanolic extracts of the samples of drying in shade,steaming with wine,stir-frying until yellow,stir-frying with bran,baking with bran,stir-frying with vinegar and with earth,with ginger juice and with rice water were in order 144.93,123.28,283.04,148.44,375.38,129.86,171.50,76.64 and 120.70 μg/mL calculated as the unprocessed.CONCLUSION The study shows that there are significant differences in the components and inhibitory activities of gastric H+/K-ATPase by different processes of Angelica polymorpha.The method of stir-frying with ginger juice on Angelica polymorpha is of good bioactive importance.