| 人轮状病毒的细胞培养分离 |
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| 引用本文： | 白植生,方悦群,吴妙灵,申硕,郑元丰,靳毅,李文琦,吴明德,赵艺.人轮状病毒的细胞培养分离[J].病毒学报,1985(2). |
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| 作者姓名： | 白植生 方悦群 吴妙灵 申硕 郑元丰 靳毅 李文琦 吴明德 赵艺 |
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| 作者单位： | 卫生部兰州生物制品研究所第二研究室
(白植生，方悦群，吴妙灵，申硕，郑元丰，靳毅，李文琦)，兰州医学院电镜室
(吴明德)，兰州医学院电镜室(赵艺) |
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| 摘 要： | 用CV-1细胞从急性腹泻病儿7份粪便标本中直接分离出4株人轮状病毒(HRV),并适应传代14代。感染细胞出现特征性细胞病变,经电镜、ELISA、免疫荧光染色及病毒RNA电泳等试验证实HRV毒株在CV-1细胞中的繁殖及抗原特异性。病毒滴度为10~(6.0)TCID_(60)。分离的4株HRV毒株均为RNA电泳型长型,经CV-1细胞传7～14代后,RNA图型与原粪样相比未见变异。
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| 关 键 词： | 人轮状病毒 CV-1细胞 |
ISOLATION OF HUMAN ROTAVIRUS IN CELL CULTURE |
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| Bai Zhisheng Fang Yuequn Wu Miaoling Shen Shuo Zheng Yuanfeng Jin Yi Li Wenqi Wu Mingde Zhao Yi Research.ISOLATION OF HUMAN ROTAVIRUS IN CELL CULTURE[J].Chinese Journal of Virology,1985(2). |
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| Authors: | Bai Zhisheng Fang Yuequn Wu Miaoling Shen Shuo Zheng Yuanfeng Jin Yi Li Wenqi Wu Mingde Zhao Yi Research |
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| Abstract: | Four human rotavirus strains were isolated from 7 fecal specimens of pediatric patients with acute diarrhea and underwent 14 passages in CV-1 cell culture.The replication and specificity of these isolates in cell culture were confirmed by characteristic cytopathic effects, EM, ELISA, IF and viral RNA electrophoresis. All of the 4 isolates were long electrophero-type and no difference in RNA patterns was observed between the original fecal extract and the strain adapted in CV-1 cell culture for 7-14 passages. |
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| Keywords: | Human rotavirus CV-1 cells |
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