| Development, chromosome location and genetic mapping of EST-SSR markers in wheat |
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| 作者姓名： | CHEN Haimei LI Linzhi WEI Xianyun LI Sishen LEI Tiandong HU Haizhou WANG Honggang ZHANG Xiansheng |
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| 作者单位： | College of Agronomy, Shandong Agricultural University/Shandong KeyLab of Crop Biology, Tai＇an 271018, China |
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| 摘 要： | A number of 151695 wheat expression sequence tags （ESTs） that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats （SSRs） with motif 2-5 bp, and 2038 simple sequence repeats （EST-SSRs）, which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework map previously constructed using recombinant inbred lines.
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| 关 键 词： | 染色体 遗传映射 小麦 序列重复 EST |
| 收稿时间： | 2005-03-15 |
| 修稿时间： | 2005-03-152005-07-28 |
Development, chromosome location and genetic mapping of EST-SSR markers in wheat |
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| CHEN Haimei LI Linzhi WEI Xianyun LI Sishen LEI Tiandong HU Haizhou WANG Honggang ZHANG Xiansheng.Development, chromosome location and genetic mapping of EST-SSR markers in wheat[J].Chinese Science Bulletin,2005,50(20):2328-2336. |
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| Authors: | Haimei Chen Linzhi Li Xianyun Wei Sishen Li Tiandong Lei Haizhou Hu Honggang Wang Xiansheng Zhang |
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| Affiliation: | 1. College of Agronomy, Shandong Agricultural University/Shandong Key Lab of Crop Biology, 271018, Tai’an, China
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| Abstract: | A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24,
2004 were used to search for simple sequence repeats (SSRs) with motif 2–5 bp, and 2038 simple sequence repeats (EST-SSRs),
which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166
amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers
in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were
located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework
map previously constructed using recombinant inbred lines. |
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| Keywords: | wheat EST-SSR primer nulli-tetrasome RIL geneticmap |
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