连钱草愈伤组织诱导及增殖研究

对连钱草Glechoma longituba (Nakai) Kupr.愈伤组织培养作了初步的探索，以不同的培养条件，利用连钱草的顶芽、叶、叶柄为外植体，研究了连钱草愈伤组织的培养。结果表明：在以MS培养基和LS培养基为基本培养基附加不同外源激素2，4-D、NAA、KT、BA条件下，连钱草在一个较宽的生长范围内，均可诱导产生连钱草的愈伤组织，但不同外植体、不同类型植物激素及其不同浓度对愈伤组织发生均有一定影响：作为外植体，连钱草叶柄和叶都可顺利诱导出愈伤组织；生长素2，4-D对连钱草外植体的脱分化起促进作用，但NAA却抑制愈伤组织的形成；细胞分裂素KT和BA均能与2，4-D组合促进愈伤组织的诱导。MS+2，4-D在光暗交替条件下和LS+2，4-D在黑暗条件下有利于连钱草愈伤组织的诱导，最佳诱导和增殖条件是MS+2，4-D（1.5 mg·L^-1）+BA（1.0 mg·L^-1）光、暗交替（光照14 h·d^-1）。在此条件下， 30 d后，叶的诱导率达91.38%，叶柄的诱导率达100%；愈伤组织继代培养14 d后，平均增殖率达202.2%。

Studies on callus induction and multiplication of Glechoma longituba (Nakai) Kupr.

The callus induction of Glechoma longituba was preliminary explored in this paper. Bud, leaf and leafstalk were cultured on different cultural conditions. The results showed that the callus of G. longituba could be induced on MS or LS medium supplemented with different kinds of hormones: 2，4-D, NAA, KT, BA. The frequency of callus induction was different when different explants were cultivated in medium supplemented with different types and different concentrations of hormones. The callus was induced by using leafstalk and leaves as explants; 2，4-D could promote to dedifferentiation, but NAA could restraint the callus to form. KT or BA mixed with 2，4-D both promoted the induction. MS+2，4-D, darkness and light in turn or LS+2，4-D,darkness were both good for callus induction, and the optimized condition for induction and multiplication was MS+2，4-D(1.5 mg·L^-1)+BA(1.0 mg·L^-1) in darkness and light in turn (14 hours illumination every day). After 30 days,the inducing rates of leaf could reach 91.38% and The inducing rates of leafstalk could reach 100%.After being subcultured for 14 days,the average multiplication rates could be up to 202.2%.