胆囊癌GBC-SD细胞经顺铂处理后的survivin表达及意义

目的: 探讨经顺铂（DDP）处理胆囊癌细胞后survivin表达及其与肿瘤细胞耐药之间的关系。 方法:采用MTT比色法测定胆囊癌细胞对4种化疗药物的敏感性。RT-PCR检测survivin mRNA的表达。Western blot检测survivin蛋白表达的变化。结果:GBC-SD细胞对化疗药物的敏感性从高到低依次为DDP>ADM>5-FU>MMC。化学药物处理后的第1天，3组胆囊癌细胞的survivin mRNA表达水平均降低;其中0.5μg/mL DDP＋GBC-SD组下降了10%，3μg /mL DDP＋GBC-SD组下降36%，6μg /mL DDP＋GBC-SD组下降了28%。第3天，0.5μg/mL DDP组和3μg/mL DDP组GBC-SD细胞的survivin mRNA表达与第1天比较，分别上升22%和64%，但6μg/mL DDP组仍持续降低，仅为第1天的66%。0.5μg/mL DDP组和3μg/mL DDP组作用3d后的GBC-SD细胞中survivin蛋白含量分别升高了15%和12%，而6μg/mL DDP组则下降了80%。 结论:低浓度的DDP即能诱导胆囊癌细胞内survivin的表达增加，这可能是胆囊癌细胞对化疗药物产生耐药性的因素之一。

The expression of survivin mRNA and protein induced by cisplatin in gallbladder carcinoma cell line (GBC-SD)

Abstract:Objective:To explore the relationship between survivin and chemoresistance of gallbladder cancer cell line (GBC-SD) cells after treatment with various concentrations of cisplatin. Methods:RT-PCR and Western blot were used to analyze the changes in expression levels of survivin mRNA and protein. GBC-SD cells were examined with MTT assay to determine drug sensitivity to 4 routine chemotherapeutic drugs. Results:In GBC-SD cells, the sensitivity sequence of those 4 drugs from high to low was DDP>ADM>5-FU>MMC. The level of survivin mRNA decreased in all the GBC-SD cells treated with three concentrations of cisplatin for one day. The survivin mRNA treated with 0.5μg/mL DDP decreased 10%, decreased 36% for 3μg/mL DDP，and decreased 28% for 6μg/mL DDP. In the third day, the survivin level treated with 0.5μg/mL DDP cells increased 22%, with 3μg/mL DDP increased 64%, but with 6μg/mL DDP decreased 34% compared with the first day. Similarly, survivin protein level increased 15% for those cells treated with 0.5μg/mL DDP after three days, increased 12% for 3μg/mL DDP, but decreased 80% for 6μg/mL DDP. Conclusions:A high expression of survivin was induced in GBC-SD by low concentration of cisplatin. Survivin may correlate with the chemoresistence of GBC-SD cells.