| 从香蕉胚性细胞悬浮系获得再生植株 |
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| 引用本文： | 徐春香,陈厚彬,李建国,张海岚,彭国康,梁铨荣.从香蕉胚性细胞悬浮系获得再生植株[J].植物生理学通讯,2004,40(2):161-163. |
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| 作者姓名： | 徐春香 陈厚彬 李建国 张海岚 彭国康 梁铨荣 |
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| 作者单位： | 华南农业大学园艺学院热带亚热带果树研究室,广州,510642 |
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| 基金项目： | 华南农业大学校科研和教改项目，广东省科研项目 |
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| 摘 要： | 2个主栽香蕉品种的未成熟雄花诱导产生的胚性愈伤组织接种至液体培养基中,经3～4个月的继代培养后长成质地均匀的胚性细胞悬浮系(ECS),悬浮系中60%～80%是胚性细胞团.ECS接种至体胚再生培养基上约4～5周后开始出现再生体胚,萌发的体胚以MS培养基培养后可获得再生植株.
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| 关 键 词： | 香蕉 胚性细胞悬浮系 植株再生 |
| 修稿时间： | 2003年5月19日 |
Plant Regeneration from Embryogenic Cell Suspension of Banana |
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| XU Chun-Xiang,CHEN Hou-Bin,LI Jian-Guo,ZHANG Hai-Lan,PENG Guo-Kang,LIANG Quan-RongTropical and Subtropical Fruit Research Laboratory,College of Horticulture,South China Agricultural University,Guangzhou.Plant Regeneration from Embryogenic Cell Suspension of Banana[J].Plant Physiology Communications,2004,40(2):161-163. |
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| Authors: | XU Chun-Xiang CHEN Hou-Bin LI Jian-Guo ZHANG Hai-Lan PENG Guo-Kang LIANG Quan-RongTropical and Subtropical Fruit Research Laboratory College of Horticulture South China Agricultural University Guangzhou |
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| Affiliation: | XU Chun-Xiang*,CHEN Hou-Bin,LI Jian-Guo,ZHANG Hai-Lan,PENG Guo-Kang,LIANG Quan-RongTropical and Subtropical Fruit Research Laboratory,College of Horticulture,South China Agricultural University,Guangzhou510642 |
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| Abstract: | Embryogenic calli derived from immature flowers of 2 banana cultivars (Musa spp., AAA Group)widely grown in China were used to establish embryogenic cell suspension (ECS). ECS was obtained aftercontinuous subculture and selection for 3~4 months, in which about 60%~80% was embryogenic cell aggregates.Regenerable somatic embryos emerged after the ECS was cultured on somatic embryo regeneration medium for4~5 weeks. Banana regenerated plants were obtained after the germinated somatic embryos were transferred toMS medium. |
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| Keywords: | banana embryogenic cell suspension plant regeneration |
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