连云港市2010年手足口病的病原学检测与分析

目的 对连云港市2010年手足口病例标本进行病原学检测,了解手足口病的感染情况,分析检测结果,为手足口病的预防和临床诊断提供科学依据.方法 采用Real Time RT-PCR方法对手足口病患者咽拭子、肛拭子标本进行肠道病毒(EV)、肠道病毒71型(EV71)和柯萨奇病毒16型(CoxA16)特异性核酸的检测.结果 475例患者标本中EV71阳性率27.8％(132例),CoxA16阳性率20.0％(95例),其他肠道病毒阳性率7.8％(37例);全年的感染高峰是4～6月份,占全年的39.58％;发病年龄以低年龄组为主,0～3岁感染率为77.68％.结论 手足口病发病有明显的时间和年龄差异,连云港市手足口病病原以EV71和CoxA16为主,重症病例的病原以EV71为主,荧光定量RT-PCR可快速诊断手足口病病原.

Etiology detection and analysis on hand-foot-and-mouth disease during 2010 in Lianyungang

OBJECTIVE To complete pathogen detection of HFMD cases samples in Lianyungang in 2010,understand the HFMD infection and to analyze test results for the hand,foot and mouth disease prevention,and provide a scientific basis for clinical diagnosis.METHODS Enterovirus(EV),Enterovirus 71(EV71)and Coxsackie virus 16(CoxA16)-specific nucleic acid in patients were collected by the Real Time RT-PCR methods and hand,foot and mouth as well as rectal swab specimens.RESULTS Among 475 cases,the positive rate of EV71 specimens was 27.8%(132 cases),the positive rate of CoxA16 was 20.0%(95 cases),and positive rate of other Enterovirus was 7.8%(37 cases);The peak month of infection was at the range of April to June months,which accountd for 39.58%;The dominated age was the low age group,and the infection rate of 0 to 3 years was 77.68%.CONCLUSION Hand,foot and mouth disease has obvious time and age difference.The main pathogenic bacteria were EV71 HFMD and CoxA16.The severe case was EV71.Quantitative RT-PCR could rapid diagnose the pathogenic bacteria of foot and mouth disease.