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Trichoderma atroviride as a bioremediator of Cu pollution: An in vitro study
Authors:Mazyar Yazdani  Chee Kong Yap  Faridah Abdullah  Soon Guan Tan
Affiliation:1. Faculty of Science, Department of Biology , Universiti Putra Malaysia , 43400 UPM, Serdang , Selangor , Malaysia mazyar.yazdani@bio.uio.no;3. Faculty of Science, Department of Biology , Universiti Putra Malaysia , 43400 UPM, Serdang , Selangor , Malaysia;4. Faculty of Biotechnology and Bimolecular, Department of Cell and Molecular Biology , Universiti Putra Malaysia , 43400 UPM, Serdang , Selangor , Malaysia
Abstract:Isolated Trichoderma atroviride from Cu-polluted river sediment at the Serdang Industrial Area was studied under in vitro conditions to understand the mechanisms that allowed the fungi to thrive in the Cu-polluted freshwater ecosystem. From this study, adsorption was recognized as the main mechanism of Cu tolerance with 50–85% adsorption during the in vitro experiment. The uptake capacity of the isolate in liquid medium ranged from 0.8 to 11.2 mg g?1 in the potato dextrose broth medium with increasing Cu concentrations from 25 to 300 mg L?1. It was found that 2.7–5.0% of Cu was lost due to washing. The high percentage of Cu adsorption and the high uptake capacity of Cu by T. atroviride suggest that it is a potential bioremediator of Cu. However, further studies are needed to confirm its practical use as a bioremediating agent for Cu under field conditions.
Keywords:Trichoderma atroviride  bioremediation  adsorption  Cu absorption
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