| B22 Glu Des-B30 Insulin： A Novel Monomeric Insulin |
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| 作者姓名： | Du HJ Shi JH Cui DF Zhang YS |
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| 作者单位： | [1]Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]Institute of Protein Research, Tongji University, Shanghai 200092, China [3]Graduate School of the Chinese Academy of Sciences Shanghai 200031, China |
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| 基金项目： | Acknowledgement We are grateful to Ms. Xiao-Xia SHA0 of Tongji University （Shanghai, China） for determining the molecular weight by mass spectroscopy. |
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| 摘 要： |
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| 关 键 词： | 胰岛素 药物制备 降糖药 生物活性 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-102006-05-16 |
B22 Glu des-B30 insulin: a novel monomeric insulin |
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| Du HJ,Shi JH,Cui DF,Zhang YS.B22 Glu des-B30 insulin: a novel monomeric insulin[J].Acta Biochimica et Biophysica Sinica,2006,38(8):537-542. |
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| Authors: | Du Hai-Juan Shi Jia-Hao Cui Da-Fu Zhang You-Shang |
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| Affiliation: | Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. |
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| Abstract: | Studies on monomeric insulin with reduced self-association are important in the development of insulin pharmaceutical preparations with rapid hypoglycemic action on patients with diabetes. Here we report a novel monomeric insulin, B22 Glu des-B30 insulin, prepared from a single chain insulin precursor with B22 Arg mutated to Glu, which was expressed in Pichia pastoris and converted to B22 Glu des-B30 insulin by tryptic digestion. It still retains 50% of the in vivo biological activity of porcine insulin and does not form a dimer even at a concentration of 10 mg/ml, showing that B22 Glu plays a key role in reducing the self- association of the insulin molecule without greatly reducing its biological activity. This novel monomeric insulin might have potential applications in the clinic. |
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| Keywords: | monomeric insulin B22 Glu des-B30 insulin self-association |
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