Automated atlas-based segmentation of NISSL-stained mouse brain sections using supervised learning |
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Authors: | O V Senyukova A S Lukin D P Vetrov |
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Affiliation: | 1.Department of Computational Mathematics and Cybernetics,Moscow State University,Moscow,Russia |
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Abstract: | The problem of segmentation of mouse brain images into anatomical structures is an important stage of practically every analytical
procedure for these images. The present study suggests a new approach to automated segmentation of anatomical structures in
the images of NISSL-stained histological sections of mouse brain. The segmentation algorithm is based on the method of supervised
learning using the existing anatomical labeling of the corresponding sections from a specialized mouse brain atlas. A mouse
brain section to be segmented into anatomical structures is preliminarily associated with a section from the mouse brain atlas
displaying the maximum similarity. The image of this section is then preprocessed in order to enhance its quality and to make
it as close to the corresponding atlas image as possible. An efficient algorithm of luminance equalization, an extension of
the well-known Retinex algorithm is proposed. A random forest is trained on pixel feature vectors constructed based on the
atlas section images and the corresponding class labels associated with anatomical structures extracted from the atlas anatomical
labeling. The trained classifier is then applied to classify pixels of an experimental section into anatomical structures.
A new combination of features based on superpixels and location priors is suggested. Accuracy of the obtained result is increased
by using Markov random field. Procedures of luminance equalization and subsequent segmentation into anatomical structures
have been tested on real experimental sections. |
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