Characterization of melanin isolated from a dark septate endophyte (DSE), Exophiala pisciphila |
| |
| Authors: | Zhan Fangdong He Yongmei Zu Yanqun Li Tao Zhao Zhiwei |
| |
| Affiliation: | (1) Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, People’s Republic of China;(2) College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China; |
| |
| Abstract: | Melanin produced by a dark septate endophyte, Exophiala pisciphila, was isolated and purified. The solubility properties, Ultraviolet–visible and Fourier transform infrared spectra of the
purified E. pisciphila melanin were similar to those of typical melanin. Inhibition of melanin production was observed when colonies exposed to
tricyclazole (1,8-dihydroxynaphthalene (DHN) melanin inhibitor), but not to kojic acid (3,4-dihydroxyphenylalanine melanin
inhibitor). Thus the E. pisciphila melanin was a member of DHN melanin family. In addition, the antioxidant activities of E. pisciphila melanin were evaluated in vitro by 1,1-diphenyl-2-picryl-hydrazyl radical-scavenging assay. E. pisciphila melanin exhibited a strong antioxidant activity. Addition of 50–350 mg L−1 Cd(II) to the medium increased the melanin production in E. pisciphila. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
