一测多评法测定人参花中7种人参皂苷含量

目的 在同时测定人参花中7种人参皂苷含量的基础上，建立7种人参皂苷成分一测多评方法，验证一测多评方法在人参花中人参皂苷含量测定上的可行性。方法 采用HPLC-UV法，以10批不同来源的人参花药材为研究对象，以人参皂苷Re为内参物测定其与人参皂苷Rg₁、Rg₂、Rb₁、Rc、Rb₂、Rd的相对校正因子，计算出各皂苷的含量，比较计算值与外标法实测值的差异。结果 人参花中6种人参皂苷Rg₁、Rg₂、Rb₁、Rc、Rb₂、Rd的相对校正因子分别为1.07、1.05、0.81、0.80、0.64、0.84，10批药材中6种人参皂苷的相对校正因子重复性良好，含量用一测多评方法测定与采用外标法测定时的实测值差异不显著。结论 在短缺人参皂苷对照品的情况下，可采用一测多评法方法，通过相对校正因子测定人参花中人参皂苷Rg₁、Re、Rg₂、Rb₁、Rc、Rb₂、Rd的含量。

Determination of seven saponins in flower buds of Panax ginseng by quantitative analysis of multi-components by single marker

Objective On the basis of simultaneous determination of seven saponins in flower buds of Panax ginseng, a method of quantitative analysis of multi-components by single marker (QAMS) for the determination of seven saponins was established, and the feasibility of the method was verified. Methods Using HPLC-UV, ten batches of dried P. ginseng flowers were used as the research object. Ginsenoside Re was used as internal reference to determine the relative correction factor of ginsenoside Rg₁, Rg₂, Rb₁, Rc, Rb₂ and Rd. The content of each component was measured by the traditional external standard method, and the difference between the calculated value and the measured value was compared to verify the feasibility and accuracy of the external standard method. Results The relative correction factors of six ginsenoside Rg₁, Rg₂, Rb₁, Rc1, Rb₂, and Rd in P. ginseng flower were 1.07, 1.05, 0.81, 0.80, 0.64, and 0.84, respectively. The relative correction factors of six ginsenosides were reproducible in the 10 batches, the determiation of QAMS were not significantly different from those measured by the external standard method. Conclusion In the case of shortage of ginsenoside reference substance, a method of QAMS can be used, the content of ginsenoside Rg₁, Rg₂, Rb₁, Rb₁, Rb₂, and Rd in flower buds of P. ginseng can be determined by relative calibration factor.