黄芪破壁饮片与传统饮片5 种有效成分在大鼠体内的药代动力学研究

目的：通过研究黄芪破壁饮片与传统饮片5 种有效成分（黄芪甲苷、毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素）在大鼠体内的药代动力学规律，进而探讨两者生物利用度的差异。方法：将大鼠随机分为两组灌胃给药：黄芪传统水煎液组和黄芪破壁饮片混悬液组（0.35 g? 100 g）；于不同时间点采集血样，血浆经蛋白质沉淀法处理后进行UPLC-MS/MS分析，测定血浆中5 种成分浓度，DAS 3.2.6 软件处理数据。结果：建立了测定大鼠血浆中5 种有效成分的UPLC-MS/MS分析方法，其线性方程、稳定性、重复性、精密度及回收率均符合生物样品分析方法的要求。5 种有效物质药时曲线均出现双峰现象，黄芪破壁饮片较黄芪传统饮片5种有效成分生物利用度提高1.4-2.5 倍。结论：实验结果表明黄芪破壁饮片混悬液与黄芪传统饮片在大鼠血浆中有相似的代谢行为，黄芪破壁饮片混悬液较黄芪传统饮片生物利用度有所提高。

Pharmacokinetics of Five Active Principles of Ultrafine Granular Powder of Radix Astragali and Decoction Pieces of Astragali Radix in Rats

Nano-sized drug delivery system is an ideal carrier for the delivery of antitumor drugs, which is able to greatlyimprove the therapeutic effect and obviously reduce the systemic toxicity. It presents promising potential as an alternativetreatment to the traditional chemotherapy. Based on the theory of traditional Chinese medicine (TCM), the antitumormulticomponent medicines possess a lot of advantages over chemotherapy drugs. However, TCM still faces several issuesdue to the poor absorption of insoluble components and the non-selective biodistribution. Recently, due to the rationalcombination of nano-sized drug delivery system and multicomponent TCM in the field of antitumor study, a variety ofTCM-based nano-drug system have been reported, which exhibited lots of advantages such as improved bioavailability,enhanced tumor targeting, optimized stability in vitro and in vivo, smart drug release and synergistic antitumor effects.These studies enrich the modernization of TCM, and offer a feasibility of antitumor treatment for clinical application. Thisreview focused on the research progress of TCM-based nano-sized drug delivery system for anticancer therapy in theperspective of target manner, and gave out our views on the current situation of TCM pharmaceutics.