| 高效液相色谱法测定含乳饮料中苯甲酸、山梨酸钾、水杨酸 |
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| 引用本文： | 田玉平,舒晓莲,顾玲玲,陈鹰.高效液相色谱法测定含乳饮料中苯甲酸、山梨酸钾、水杨酸[J].化学世界,2012,53(1):24-25,30. |
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| 作者姓名： | 田玉平 舒晓莲 顾玲玲 陈鹰 |
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| 作者单位： | 上海市计量测试技术研究院,上海,201203 |
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| 基金项目： | 上海市科学技术委员会资助项目 |
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| 摘 要： | 建立了含乳饮料中苯甲酸、山梨酸钾等添加剂的高效液相色谱仪同时检测的方法。含乳饮料经前处理后,由C18反相色谱柱进行分离,流动相A:磷酸盐缓冲溶液(1.25 g K2HPO4与1.25 gKH2PO4加水定容至500 mL),流动相B:乙腈,流动相A、B按9∶1比例洗脱,流速0.6 mL/min,进样量为10μL。苯甲酸检测波长为225 nm,山梨酸钾的检测波长为240 nm,水杨酸的检测波长为225 nm。该方法线性范围为0.6~30μg/mL,检测限为0.06μg/mL。
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| 关 键 词： | 含乳饮料 苯甲酸 山梨酸 水杨酸 高效液相色谱法 |
Determination of Benzoic Acid, Potassium Sorbate, Salicylic Acid in Milk Beverage by HPLC-DAD |
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| TIAN Yu-ping
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SHU Xiao-lian
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GU Ling-ling
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CHEN Ying.Determination of Benzoic Acid, Potassium Sorbate, Salicylic Acid in Milk Beverage by HPLC-DAD[J].Chemical World,2012,53(1):24-25,30. |
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| Authors: | TIAN Yu-ping SHU Xiao-lian GU Ling-ling CHEN Ying |
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| Affiliation: | (Shanghai Institute of Measurement and Testing Technology,Shanghai,201203,China) |
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| Abstract: | A HPLC method for the determination of benzoic acid,potassium sorbate,salicylic acid in milk beverage was established and reported.The sample was extracted and the components were separated on a C18 reversed phase column eluated with a mobile shase of Acn/water(1.25 g K2HPO4 and 1.25 g KH2PO4 dissolved in 500 mL water),and detected by the DAD detector.Linearity of the calibration curve was in the range of 0.6~30.0 μg/mL,and the detection limit was 0.06 μg/mL.The method was already applied to real sample analysis. |
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| Keywords: | milk beverage benzoic acid potassium sorbate salicylic acid HPLC |
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