阻断协同刺激分子对大鼠母胎界面中Caspase-3、LamininB的表达及妊娠结局的影响

目的探讨阻断协同刺激分子CD86对自然流产模型孕鼠母胎界面Caspase-3和Laminin B的表达及妊娠结局的影响。方法实验组于妊娠第4.5天腹腔注射大鼠抗小鼠CD86单克隆抗体,对照组注射大鼠同型IgG2b,而正常妊娠组不作任何处理。于妊娠第13.5天计算胚胎吸收率,用免疫组化法测定Caspase-3和Laminin B的表达。结果实验组的胚胎吸收率显著低于对照组(x2=7.441,P<0.05);实验组中Caspase-3蛋白阳性表达率明显低于对照组(P<0.05),实验组中Laminin B蛋白阳性表达率明显高于对照组(P<0.05)。结论妊娠早期阻断协同刺激分子CD86可使母胎界面中的Caspase-3和Laminin B分别通过各自不同的途径来发挥免疫耐受作用,并使自然流产模型孕鼠的胚胎吸收率降低至正常妊娠水平。

Effect of Blockade of Co- stimulatory Signal CD86 on the Expression of Caspase-3 and Laminin B Proteins and Pregnancy Outcome

Objective: To investigate the effect of blockade of co-stimulatory signal CD86 on the expression of Caspase-3 and Laminin B proteins at the maternal-fetal interface and pregnancy outcome in abortion-prone murine model. Methods: Female pregnant CBA/J mice of experimental group and control group were received an i.p injection of 100μg of anti-mouse CD86 mAb in 200 μl PBS and irrelevant-isotype matched rat IgG2b, respectively, on the day 4.5 of gestation, however, no treatment was done for the normal pregnancy group. Pregnant CBA/J mice were killed on the day 13.5 of gestation. The embryo resorption rate was calculated, and immunohistochemical method was employed to detect the expression of Caspase-3 and Laminin B proteins. Results: (1)The embryo resorption rate in experimental group was significantly reduced in comparison with that in control group(x2=7.441, P<0.05).(2)The positive expression rate of Caspase-3 in experimental group was significantly reduced in comparison with that in control group (P<0.05). The positive expression rate of Laminin B in experimental group was significantly increased in comparison with that in control group (P<0.05). Conclusion: Blockade of co-stimulatory signal CD86 at the early stage of gestation can induce Caspase-3 and Laminin B to exert immunologic tolerance by their different mechanisms and reduce the embryo resorption rate to normal pregnant level in abortion-prone murine model.