IL-33在类风湿关节炎合并间质性肺炎患者中的表达及意义

目的探讨类风湿关节炎合并肺间质病变(RA-ILD)患者的临床血清中白介素-33(IL-33)表达,特征外周血单个核细胞(PBMC)。方法类风湿关节炎(RA)患者115例,分为RA-ILD组(29例)和RA组(86例),另选取健康自愿者20例为对照组,类风湿因子(RF)、抗环瓜氨酸抗体(anti-CCP)及抗角蛋白抗体(AKA)的浓度,检测PBMC中IL-33 mRNA表达,同时通过酶联免疫吸附法(ELISA)测定各组血清中IL-33的表达。结果与RA患者比,RA-ILD组除性别外平均年龄、病程比较差异均有统计学意义,RF、anti-CCP及AKA的浓度在RA-ILD与RA两组之间无统计学差异(P0.05),RA-ILD组PBMC中IL-33 mRNA及血清中IL-33表达含量明显比RA及正常对照组升高(P0.01)。结论 RA-ILD易发生于年龄大、病程较长的RA患者,一般实验检查不能预测RA患者ILD发生的可能性;PBMC和血清中IL-33可能与RA-ILD的发病有关;IL-33水平增高可能为RA-ILD的预测指标。

Expression and significance of IL-33 in patients with rheumatoid arthritis complicated with interstitial lung disease

Objective To study the expression of IL-33 in patients with rheumatoid arthritis complicated with interstitial lung disease. Methods 115 rheumatoid arthritis （ RA） patients were divided into the RA-ILD group （29 cases） and the RA group （86 cases）, and 20 healthy people were selected as the control group. The concentra-tion of RF, anti-CCP and AKA was detected in each group. Meanwhile, the expression of IL-33mRNA in peripheral＆amp;nbsp;blood mononuclear cells （ PBMC） was detected by RT-PCR and the expression of IL-33 in serum was detected by en-zyme-linked immunosorbent assay （ ELISA） . Results There were significant differences in mean age and course of disease between the RA-ILD group and the RA group, except gender. The concentration of RF, anti-CCP and AKA had no significant difference between the two groups （P〉0. 05）. However, the expression of IL-33 mRNA in PBMC and serum level of IL-33 in the RA-ILD group were significantly higher than those in the RA group and the control group （P〈0. 01）. Conclusion RA-ILD easily occurs in elderly RA patients with longer course of disease. The general clinical laboratory tests can not predict the incidence of RA patients with ILD. IL-33 in PBMC and serum may be related to RA-ILD, and the high expression of IL-33 can be a predictor of RA-ILD.