| PGE_1对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用 |
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| 引用本文： | 宋丽杰,付润芳,王振基,冯国清,翁世艾.PGE_1对培养乳鼠心肌细胞缺氧/复氧损伤的保护作用[J].中国药理学通报,2003,19(10):1140-1142. |
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| 作者姓名： | 宋丽杰 付润芳 王振基 冯国清 翁世艾 |
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| 作者单位： | 郑州大学医学院药理学教研室,郑州,450052 |
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| 摘 要： | 目的 研究PGE1对纯化培养心肌细胞缺血再灌注损伤的保护作用及机制。方法 采用纯化培养的心肌细胞建立缺氧 /复氧损伤模型 ,实验分 5组 :正常细胞培养组、缺氧 /复氧损伤模型组、缺氧 /复氧损伤 +PGE1(5、15、4 5 μg·L-1)组。分别用黄嘌呤氧化酶法测定SOD活力 ,硫代巴比妥酸显色法测定MDA含量 ,MTT染色法测定线粒体脱氢酶活性改变并测定LDH含量变化。结果 PGE1可明显提高SOD、LDH活性 ,降低MDA含量并可提高线粒体脱氢酶活性。结论 PGE1具有明显的抗缺氧复氧损伤、保护心肌作用
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| 关 键 词： | 前列腺素E1 培养 心肌细胞 缺氧复氧损伤 |
| 文章编号： | 1001-1978(2003)10-1140-03 |
| 修稿时间： | 2003年2月18日 |
Protective effects of prostaglandin E1 on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells |
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| SONG Li Jie,FU Run Fang,WANG Zhen Ji,FENG Guo Qing,WENG Shi Ai.Protective effects of prostaglandin E1 on hypoxia/reoxygenation injury in cultured neonatal rat myocardial cells[J].Chinese Pharmacological Bulletin,2003,19(10):1140-1142. |
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| Authors: | SONG Li Jie FU Run Fang WANG Zhen Ji FENG Guo Qing WENG Shi Ai |
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| Abstract: | AIM To study the protective effects of prostaglandin E 1 on hypoxia/reoxygenation injury in cultured neonatal rat myocytes. METHODS The hypoxia/reoxygenation injury model of cultured neonatal rat myocardial cells was developed. The activities of plasma superoxide dismutase (SOD) were measured by the method of xanthine oxidase and the contents of serum malonicaldehyde (MDA) were determined by the method of thiobarbituric acid. The activities of dehydrogenase in mitochondria were detected by MTT assay. The activities of LDH in culture were also evaluated. CONCLUSION PGE 1 has protective effects on the cultured neonatal rat myocardial cells injured by hypoxia/reoxygenation. The mechanisms are related to its antioxidation effects. |
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| Keywords: | prostaglandin E 1 culture myocardial cell hypoxia/reoxygenation |
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