| 非标记金纳米粒子共振光散射法检测汞离子 |
| |
| 引用本文： | 周斌,史林飞,刘璐,王永生.非标记金纳米粒子共振光散射法检测汞离子[J].应用化工,2012,41(2):344-346. |
| |
| 作者姓名： | 周斌 史林飞 刘璐 王永生 |
| |
| 作者单位： | 南华大学公共卫生学院,湖南衡阳,421001 |
| |
| 基金项目： | 湖南省科技厅科技计划资助项目(2010SK3039) |
| |
| 摘 要： | 在pH 7.3的磷酸盐缓冲溶液中,汞特异性寡核苷酸与汞结合,使金纳米粒子在高盐浓度下聚集,导致体系的共振光散射增强,在4.4×10-8～5.0×10-7 mol/L范围内,散射光强度的改变值与汞离子浓度呈良好线性关系,回归方程为ΔIRLS=0.756+119.94 c(×10-7 mol/L),r2=0.998,检出限为1.3×10-8 mol/L。用于环境水样检测,回收率在96.4%～106.8%,该方法简便、快速、选择性好、灵敏度高。
|
| 关 键 词： | 汞 汞特异性DNA 共振光散射 |
Resonance light scattering detection of mercury ( Ⅱ ) ion using unlabelled AuNPs |
| |
| ZHOU Bin
,
SHI Lin-fei
,
LIU Lu
,
WANG Yong-sheng.Resonance light scattering detection of mercury ( Ⅱ ) ion using unlabelled AuNPs[J].Applied chemical industry,2012,41(2):344-346. |
| |
| Authors: | ZHOU Bin SHI Lin-fei LIU Lu WANG Yong-sheng |
| |
| Affiliation: | (College of Public Health,University of South China,Hengyang 421001,China) |
| |
| Abstract: | In phosphate buffer solution of pH 7.3,mercury specific oligonucleotide(MSO) can combine with mercury to form AuNPs aggregates upon addition of NaCl solution,resulting in the enhancement of resonance light scattering(RLS) intensity.The RLS intensity linearly correlated with the concentration of mercury over the range of 4.4 × 10-8～5.0 × 10-7 mol/L,and the linear regression equation was ΔIRLS = 0.756 + 119.94 c(×10-7 mol/L),r2 = 0.998.The limit of detection(LOD) for mercury was 1.3×10-8 mol/L,and the recovery was 96.4% ~ 106.8%.The proposed method was simple,rapid,sensitive and selective. |
| |
| Keywords: | mercury mercury specific oligonucleotide resonance light scattering |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
