益精方对腺嘌呤不育症大鼠睾丸支持细胞间连接黏附分子和闭合小环蛋白表达的作用

目的观察益精方对腺嘌呤不育症肾阳虚大鼠睾丸支持细胞间连接黏附分子(JAM)及闭合小环蛋白(ZO)表达的影响。方法选用48只健康的SPF级Wistar雄性大鼠,2月龄,按随机数字表法,平均分为正常组、模型组、复方玄驹组、益精方组,每组12只。造模成功后,各给药组灌胃相应药物,连续20 d。然后处死大鼠,摘取睾丸和附睾,采用计算机精子分析系统计数精子密度和精子活动率;采用免疫组化方法测定大鼠睾丸支持细胞间JAM及ZO表达。结果与正常组比较,模型组精子密度、精子活动率、JAM及ZO表达均显著降低(P0.01)。与模型组比较,复方玄驹组和益精方组精子活动率、精子密度均显著升高(P0.05),益精方组JAM及ZO表达显著增加(P0.05)。结论益精方能够通过调控JAM及ZO的表达,修复腺嘌呤法诱导的肾阳虚模型大鼠的睾丸支持细胞紧密连接蛋白的损伤。

Effect of Yijing Fang on expression of junctional adhesion molecules and zonula occludens in testicular sertoli cells of adenine-induced infertile rats

Objective To observe the effect of Yijing Fang,a TCM compound,on the expression of junctional adhesion molecules (JSM)and zonula occludens (ZO)in testicular sertoli cells of infertile rats with kidney-yang deficiency pattern induced by adenine.Methods Altogether 48 healthy male Wistar rats of SPF grade,two-month-old,were divided according to random number table,into blank group, model group,Fufang Xuanju group and Yijing Fang group(n =12 each).After modeling,the rats were intragastrically administered by corresponding medicines for consecutive 20 days.Then the rats were sac-rificed and their testis and epididymis were resected.The sperm density and sperm activity were calcula-ted using Computer Assisted Sperm Analysis System,and the expression of JAMand ZO in testicular ser-toli cells were measured using immunohistochemical method.Results Compared with the normal group, the sperm density,sperm activity rate and expression of JAMand ZO declined significantly(P <0.01). Compared with the model group,the sperm density and the sperm activity rate increased in Fufang Xuan-Ju group and Yijing Fang group remarkably (P <0.05),while expression of JAM and ZO increased in Yijing Fang group((P <0.05 ).Conclusion Yijing Fang can repair the damage of testicular sertoli cells of kidney-yang deficiency pattern rats induce by adenine,via regulating the expression of JAM and ZO.