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| 移植后的Wistar大鼠骨髓间充质干细胞向神经元分化的实验研究 | |
| 引用本文： | 陈玉丙,贾全章,李东君,孙景海,吴传真,王风华,徐爽,刘丽萍,姜大伟,高德萱,张红梅.移植后的Wistar大鼠骨髓间充质干细胞向神经元分化的实验研究[J].中国实验诊断学,2011,15(8):1249-1251. |
| 作者姓名： | 陈玉丙 贾全章 李东君 孙景海 吴传真 王风华 徐爽 刘丽萍 姜大伟 高德萱 张红梅 |
| 作者单位： | 1. 吉林大学第二医院,吉林,长春130041 2. 解放军第208医院 3. 吉林大学药学院 4. 深圳市东湖医院 |
| 基金项目： | 吉林省科技发展计划项目，吉林省自然科学基金项目 |
| 摘 要： | 目的观察Wistar大鼠骨髓间充质干细胞（BMSCs）移植后在脊髓损伤区是否向神经元分化。方法首先用BrdU在体外标记BMSCs,将标记后的BMSCs分别由大鼠尾静脉及脊髓损伤局部移植到大鼠体内,于移植后10天、20天及30天灌杀大鼠,制作脊髓的冰冻切片并行免疫组织化学染色,在激光共聚焦显微镜下观察植入的BMSCs表达神经元特异性蛋白NSE的情况。结果移植术后10天2、0天及30天,损伤脊髓内可见BrdU/NSE双标记的细胞。结论 BMSCs移植后在脊髓损伤区内可见神经元特异性蛋白NSE,移植的BMSCs可能分化为神经元。 |
| 关 键 词： | 骨髓间充质干细胞 神经元 分化 |
Experimental study of Wistar rats' bone marrow mesenchymal stem cells which were transplanted would transform to neurons |
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| CHEN Yu-bin,JIAN Quan-zhuang,LI Dong-jun,et al..Experimental study of Wistar rats' bone marrow mesenchymal stem cells which were transplanted would transform to neurons[J].Chinese Journal of Laboratory Diagnosis,2011,15(8):1249-1251. | |
| Authors: | CHEN Yu-bin JIAN Quan-zhuang LI Dong-jun |
| Affiliation: | CHEN Yu-bin,JIAN Quan-zhuang,LI Dong-jun,et al.(Department of Radiotherapy,Second Hospital,Jilin University,Changchun 130041,China) |
| Abstract: | Objective To transplant Wistar rats' bone marrow mesenchymal stem cells(BMSCs) into the rats' spinal cord injury zone.observe the BMSCs Whether or not to differentiate into neurons.Methods First of all,marker BMSCs with BrdU in vitro,the BMSCs will be transplanted respectively by the rat tail vein and partial spinal cord injury in rats.By the 5th days,10th days,15th days after the transplantation,these rats are perfused to kill for the spinal cords.The spinal cords are maked into frozen section,which are ex... |
| Keywords: | Bone marrow-derived mesenchymal stem cells neuron differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
