| 超高效液相色谱法测定四乙酰葛根素的含量及有关物质 |
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| 引用本文： | 胡人月,张毅,陈国庆,杜洪飞,杨大坚.超高效液相色谱法测定四乙酰葛根素的含量及有关物质[J].中南药学,2012,10(3):196-198. |
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| 作者姓名： | 胡人月 张毅 陈国庆 杜洪飞 杨大坚 |
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| 作者单位： | 重庆市中药研究院,重庆,400065 |
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| 基金项目： | 国家科技部重大专项项目 |
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| 摘 要： | 目的 建立测定四乙酰葛根素的含量及有关物质的超高效液相色谱法.方法 采用Agilent XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈-水(45∶55),流速:1.2 mL·min-1,检测波长:250 nm,柱温:30℃.结果 四乙酰葛根素的浓度在0.020 76～0.186 8mg·mL-1与峰面积线性关系良好(A=1.287×104 C-24.55,r=0.999 9,n=7),检测限为6.65 ng ·mL-1(S/N=3)；平均加样回收率为99.9％ (RSD=1.4％,n=9)；有关物质含量为1.02％～1.28％.结论 该方法简便、准确,可用于四乙酰葛根素的含量及有关物质的测定.
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| 关 键 词： | 四乙酰葛根素 超高效液相色谱法 含量测定 有关物质 |
UPLC determination of the content and related substances of tetra-acetylated puerarin |
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| HU Ren-yue
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ZHANG Yi
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CHEN Guo-qing
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DU Hong-fei
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YANG Da-jian.UPLC determination of the content and related substances of tetra-acetylated puerarin[J].Central South Pharmacy,2012,10(3):196-198. |
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| Authors: | HU Ren-yue ZHANG Yi CHEN Guo-qing DU Hong-fei YANG Da-jian |
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| Affiliation: | (Chongqing Academy of Chinese Materia Medica,Chongqing 400065) |
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| Abstract: | Objective To establish a UPLC method to determine the content and related substances of tetra-acetylated puerarin.Methods Agilent XDB-C18 chromatographic column(4.6 mm×250 mm,5 μm)was used and the mobile phase consisted of acetonitrile-water(45∶55)with a flow rate of 1.2 mL·min-1.The UV detection wavelength was 250 nm.The column temperature was 30 ℃.Results The calibration curve was linear over 0.020 76-0.186 8 mg·mL-1(A=1.287×104C-24.55,r=0.999 9,n=7).The detection limit was 6.65 ng·mL-1(S/N=3).The average recovery was 99.9%(RSD=1.4%,n=9).The content of related substances of tetra-acetylated puerarin was over 1.02%-1.28%.Conclusion The method is simple,accurate,and applicable for the quality control of tetra-acetylated puerarin. |
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| Keywords: | tetra-acetylated puerarin UPLC content determination related substance |
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