应激引起大鼠脑卒中时脑组织细胞与机体防御基因的表达差异

目的探讨应激引起大鼠脑卒中时细胞与机体防御基因的表达差异以及在发病学中的意义。方法应用冷刺激加高盐饮食复合刺激因素作用于Wistar大鼠,诱发大鼠高血压脑卒中作为模型,应用抑制性差减克隆技术对卒中样发作鼠和正常对照鼠脑的差异基因进行筛选。经过两轮杂交后,产生的克隆均为脑卒中鼠脑组织特异表达的序列。每组随机挑取288个克隆,进行测序及GenBankBlast生物信息分析。结果两组共有456个可用序列,经对每个与已知基因高度同源的序列以功能为参照进行分类,发现大鼠卒中后脑组织细胞与机体防御基因表达明显下调(P<001),卒中组的288个克隆中未发现运输蛋白/膜转运及应激反应相关基因的表达。结论脑组织运输蛋白/膜转运及应激反应相关基因的表达发生改变,可能是脑卒中发生的分子机制之一。

Differential expression of genes for cell/organism defense in cerebral cells of rats with stroke episode induced by stress

Objective:Stress has been implicated in the pathogenesis of stroke. The mediator of stress that induces cerebrovascular diseases and its roles in neuroprotection for cells and tissues during stress injury is unclear yet. It is explored that the differential expression of genes for cell/organism defense in cerebral cells of rats with stroke-like episode induced by stress in this study. Methods: A model of hypertension rats was established with cold-stimulation plus high-salt intake for 8 weeks, which were used as environment risk factors. The stroke episode appeared in some animals within 6 to 8 weeks and then their brains were taken out. The differential genes between the stroke animals and the normal animals were identified by suppression subtractive hybridization(SSH). 288 clones were selected randomly from each group and their gene sequences were measured. These sequences were compared with those in GenBank. Results: A total 576 clones were obtained from two subtractive libraries and 456 genes among them were usable. It was found that the expression of genes for cell/organism defense in the cerebral cells of stroke rats was down-regulation after stroke(p<0.01), compared with that of the normal rats. Genes for carrier proteins/membrane transport and stress response had not been detected in stroke group. Conclusion: It is suggested that genes for carrier proteins/membrane transport and stress response may increase the potential to stroke through the changes of gene expression. Down-regulation of the genes for cell/organism defense maybe play an important role in the mechanism of stroke.