The production of reactive oxygen species in peripheral blood neutrophils is modulated by airway mucous |
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Authors: | Agne Babusyte Jolanta Jeroch Rimantas Stakauskas Raimundas Sakalauskas |
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Affiliation: | (1) Laboratory of Pulmonology, Institute for Biomedical Research, Kaunas University of Medicine, LT-50009 Kaunas, Lithuania;(2) Department of Laboratory Medicine, Kaunas Medical University Hospital, LT-50009 Kaunas, Lithuania;(3) Institute of Immunology, Universtiy of Veterinary Medicine, D-30173 Hannover, Germany;(4) Department of Pulmonology and Immunology, Kaunas Medical University Hospital, LT-50009 Kaunas, Lithuania |
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Abstract: | Neutrophils are a major source of reactive oxygen species (ROS). The role of airway mucous on ROS production is unknown. The
aim of our study was to investigate the direct influence of bronchoalveolar lavage fluid (BALF) and induced sputum (IS) alone
or in combination with chemical/biological stimulus on ROS production in peripheral blood neutrophils during chronic obstructive
pulmonary disease (COPD). Neutrophils were isolated from peripheral blood of 47 patients with moderate COPD and 14 healthy
individuals (HI). BALF/RPMI (1:1) or IS/RPMI (1:1) from COPD patients were used to stimulate neutrophils alone or in combination
with phorbolmyristate- acetate (PMA) (0.1–30 nM) or Staphylococcus aureus bacteria (0.7–500 bact/neutrophil). Relative generation
of ROS was measured flow cytometrically. BALF/RPMI and in combination with relatively low PMA or all bacteria concentrations
stimulated ROS; while, combination with relatively high PMA concentrations suppressed ROS in of COPD patients and HI. IS/RPMI
and its combination with PMA inhibited ROS generation in both groups; whereas, IS stimulated or had a tendency to stimulate
ROS production with relatively high bacteria concentrations. In conclusion, BALF and IS directly or in combination with chemical/biological
factors modulated ROS production. This effect was stronger in neutrophils from COPD patients and depended on chemical/biological
stimulus intensity. |
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Keywords: | BALF COPD IS Neutrophils ROS |
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