大黄素和环孢素A在抗大鼠肝移植排斥反应中的协同作用

目的 探讨大黄素与环孢素A合用对大鼠肝移植排斥反应的影响。 方法 以LEW→BN大鼠原位肝移植为模型，随机分为4组，每组12只，术后每天分别腹腔注射生理盐水0.5 mL 、环孢菌素A(CsA) 10.0 mg/kg、大黄素50.0 mg/kg和大黄素50.0 mg/kg+ CsA10.0 mg/kg。给药7天，于术后第8天每组随机取6只鼠处死，取肝脏组织观察排斥活动指数（RAI）和肝细胞凋亡指数（AI）；剩余大鼠停止用药，观察存活时间。 结果 CsA组、大黄素组、大黄素+ CsA组的存活天数、RAI及AI优于生理盐水组（P<0.01），其中大黄素+ CsA组优于CsA组、大黄素组（P<0.05），CsA组和大黄素组间比较差异无统计学意义（P>0.05）。 结论 大鼠肝移植术后给予大黄素与环抱素A联用，对于抑制排斥反应有协同作用。

Synergistic Effect of Emodin and Cyclosporine A on Rejective Reaction against Liver Graft in Rats

OBJECTIVE: To evaluate the effect of emodin in combination with cyclosporine A (CsA) on rejective reaction against liver graft in rats. METHODS: The LEW-->BN orthotopic liver transplantation rat model was used in the study. A total of 48 rats were divided into 4 groups randomly and equally, after operation they were intraperitoneally injected respectively with normal saline (0.5 mL d(-1), group A); CsA (10.0 mg kg(-1) d(-1), group B); emodin (50.0 mg kg(-1) d(-1), group C); and CsA plus emodin (group D, at the same dose as in B and C). Six rats taken from each group were sacrificed on the 8th day after operation to calculate the rejection active index (RAI) and hepatocyte apoptosis index (AI). The remainder were stopped medication and used for observing the survival time. RESULTS: The inter-group comparisons in mean survival time, RAI and AI showed significant difference in comparing group A with group B, C and D (P <0.01), and those in group D were more obvious than in group B and C (P < 0.05, but showed no significant difference between group B and group C (P > 0.05). CONCLUSION: Administering of emodin combined with CsA after liver transplantation shows a synergistic effect for suppressing acute rejective reaction in rats.