血清标本10546份非梅毒螺旋体抗原血清学试验假阳性结果分析

目的了解非梅毒螺旋体抗原血清学试验假阳性发生率及原因。方法对本院2007-2008年门诊及住院病人送检的血清标本进行快速血浆反应素(RPR)环状卡片试验和梅毒螺旋体颗粒凝集试验(TP-PA),对结果进行统计学分析。结果 2007-2008年合计检测了10546份血清标本,RPR假阳性率为0.21%(15/7002)。2007年(0.32%)与2008年(0.11%)的RPR假阳性率差异无统计学意义(P0.05)。15例RPR假阳性血清标本中10例为有高危性行为的常规检查者,2例为孕妇,皮肌炎、尖锐湿疣和龟头破溃者各1例。结论 RPR假阳性有一定的发生率。建议RPR阳性者以TPPA等梅毒螺旋体抗原血清学试验进行确认。

The False Positive Non-treponemal Antigen Serologic Test in 10 546 Sera Samples

Objective To investigate the incidence and reasons of false-positive results in rapid plasma reagin(RPR)test.Methods The sera samples were tested with both RPR and Treponema pallidum particle assay(TPPA)from STD clinic clients,pre-admission and pre-operation patients in 2007 and 2008.Results A total of 10546 sera samples were tested.The total false positive RPR rate was 0.21%(15/7002).No significant difference of the false positive rates was observed between 2007 and 2008(P>0.05).Among 15 sera samples with false positive RPR,ten from routine check-up had high-risk sex behaviors,two were pregnant women,and 1 was dermatomyositis,genital warts or glans ulcer patients respectirely.Conclusions There is a certain false positive rate of RPR in clinical settings.It is suggested that patients with positive RPR be confirmed by specific syphilitic serologic test(i.e.,TPPA)while making diagnosis.