| 糖尿病患者血清中GAD-Ab、IAA、IL-6、TNF-α测定的意义 |
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| 引用本文： | 任增芳.糖尿病患者血清中GAD-Ab、IAA、IL-6、TNF-α测定的意义[J].医学检验与临床,2011,22(2):10-12. |
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| 作者姓名： | 任增芳 |
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| 作者单位： | 山东医学高等专科学校附属医院检验科,山东临沂,276004 |
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| 摘 要： | 目的 观察细胞因子白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）在糖尿病（DM）患者发病过程中的变化,以及谷氨酸脱羧酶抗体（GAD-Ab）和胰岛素自身抗体（IAA）的存在状况.方法 采用放射免疫分析（RIA）法和免疫放射分析（IRMA）法,对200例DM患者和50例正常对照者血清中IL-6、TNF-α、GAD-Ab和IAA进行检测.结果 DM患者血清IL-6和TNF-α含量均较正常健康者高（P＜0.05）.在DM1与DM2两组中,DM1组IL-6值显著高于DM2组和正常对照组（P均＜0.01）;TNF-α在DM1与DM2两组中其含量均高于正常对照组,但以DM2组较为显著（P＜0.05）.在DM患者中,DM1组GAD-Ab的阳性检出率为75.0%,明显高于DM2组和正常对照组（P＜0.01）;DM2组IAA阳性率高于DM1组（P＞0.05）.结论 IL-6和TNF-α在DM的发病机制中有十分重要的意义,检测DM患者血清IL-6和TNF-α含量,对于判断DM患者的免疫状态,以及胰岛和胰岛素的功能状态具有重要意义.GAD-Ab、IAA联合检测对于提高DM诊断的准确性和自身免疫性1型DM的检出率、减少误诊率与漏诊率具有重要价值.
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| 关 键 词： | 糖尿病 白细胞介素-6 肿瘤坏死因子-α 谷氨酸脱羧酶抗体 胰岛素自身抗体 |
The significance of serum GAD-Ab,IAA,IL-6 and TNF-α detection in DM patients |
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| Ren Zengfang.The significance of serum GAD-Ab,IAA,IL-6 and TNF-α detection in DM patients[J].Medical Laboratory Science and Clinics,2011,22(2):10-12. |
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| Authors: | Ren Zengfang |
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| Affiliation: | Ren Zengfang(Clinical laboratory of affiliated Hospital of Shandong medical College Linyi, Shandong 276004) |
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| Abstract: | Objective To investigate the pathogenesis change of cytokine interleukin -6 （IL- 6） and tumor necrosis factor- α（TNF- α） in diabetes mellitus （DM）, and to investigate the existing condition of glutamic acid decarboxylase antibody （GAD - Ab） and insulin autoantibodies（IAA）. Melhods Serum levels of IL- 6, TNF- α, GAD- Ab and IAA were detected by radioinmmnoassay （RIA） and immunora- diometric assay methods （IRMA） in 200 cases of DM patients and 50 normal controls. Results Serum levels of IL- 6 and TNF- a in DM patients were higher than those in healthy （P 〈 0.05）. Serum level of IL - 6 in DMI group was significantly higher than that in DM2 group and control group （P 〈 0.01 all） ; Serum level of TNF - α in DM1 and DM2 groups were higher than that in control group, but that in DM2 group was high significantly （P 〈 0.05）. Positive rate of GAD - Ab in DM1 group was 75.0%, which significantly higher than those in DM2 group and normal control group （P 〈 0.01 ）; IAA- positive rate in DM2 group was higher than that in DM1 group （P 〉 0.05）. Conclusion It was important to study the pathogenesis of DM by detecting IL - 6 and TNF - α. Levels of IL - 6 and TNF - α could be used as judging the patient＇ s immune status, functional status of insulin and insulin. Combined detection of GAD- Ab and IAA could improved the accuracy of diagnosis of autoimmune type 1 DM, reduced the misdiagnosis rate and the rate of misdiagnosis. |
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| Keywords: | Diabetes meUitus Interleukin- 6 Tumor necrosis factor- α Glutamic acid decarboxylase antibody Insulin autoantibodies |
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