2005-2008年兰州地区婴幼儿腹泻腺病毒感染的分子流行病学调查

目的 通过分子流行病学调查研究兰州地区婴幼儿腺病毒(AdV)腹泻特点.方法 采用聚合酶链反应(PCR)对2005年7月-2008年6月兰州地区婴幼儿腹泻粪便标本进行AdV检测.结果 889份标本中共检出AdV的感染43例,总阳性率是4.8%,其中肠道腺病毒(EAdV,F亚属)Ad40,Ad41阳性率3.8%(34/889),其次是非肠道腺病毒(NEAdV)Ad12,Ad18,Ad31,Ad2,Ad5,Ad6,Ad7阳性率1.0%(9/889).发病年龄高峰以1岁内的婴幼儿为主,呈全年散发流行,未表现出明显的季节性.结论 AdV是兰州地区婴幼儿病毒性腹泻的重要病原之一,其主要的流行株为Ad41型.

Molecular epidemiology of human adenovirus diarrhea among infants and young children in Lanzhou from July 2005 to June 2008

Objective Gastroenteritis is a major cause of childhood morbidity and mortality worldwide.Adenovirus AdV is recognized to be one of the most important pathogens associated with severe dehydrating gastroenteritis.Studies reported elsewhere have shown that about 8%-10% of cases with infantile diarrhea are caused by AdV and in some areas AdV diarrhea even occurred in the form of outbreaks.Studies have confirmed that AdV infections are also very common in infants and young children in China.This study aimed to investigate the molecular epidemiologic characteristics of human adenovirus diarrhea among infants and children with acute diarrhea in Lanzhon,Gansu province,China.Method Stool specimen and case information were collected from both outpatients and inpatients with acute diarrhea in Lanzhou.Polymerase chain reaction was used to detect AdV in stool specimens.The subjects included 709 urban children and 180 rural children,their age ranged from 19 d to 60 months.Result Of the 889 cases,43(4.8%)were found positive for AdV.AdV was detected in 14 of 257(5.4%)cages seen from July 2005 to June 2006,in 4 of 286 cases(1.4%)seen from July 2006 to June 2007.During the period of July 2007 to June 2008,adenovirus was detected in 346 specimens,the positive rate was 7.2%(25/346).AdV detection rates of the three-year period were significantly difierent.The major AdV subtypes detected were adenovirus(subgenus F)Ad40,Ad4l with a positive rate of 3.8%(34/889),followed by non-enteric adenovirus(Ad12,Ad18,Ad31,Ad2,Ad5,Ad6,Ad7)with a positive rate of 1.0%(9/889)in Lanzhou during the 3 years.Most of the AdV-positive specimens showed Ad41 group F(67.4%,29/43)as the major epidemic strains,and Ade infection mainly occurred in children under one year of age and no seasonal cluster was found.Conclusion Adenovirus was one of the major etiological agent of viral diarrhea among infants and children in Lanzhou between 2005 and 2008.Ad41 was the prodomiment serotype.