与细胞增殖相关的核仁杭原

用自制的抗核仁抗原的抗血清对其相应的核仁抗原(NAg-1)进行了研究.间接免疫荧光染色及细胞化学分析表明,NAg-1,可能是一种与DNA结合并与rDNA合成有关的酸性蛋白质.其在静止的人淋巴细胞和人正常非增殖组织中基本不表达或仅有微量表达,在人癌细胞和人正常增殖细胞中表达.并具有一定的种属特异性.NAg-1在快速增殖的HL-60细胞中表达的百分比大大高于缓慢增殖的细胞.随着HL-60细胞的密度增大,其细胞核仁抗原表达的百分比大大下降,说明NAg-1与细胞的增殖相关.

NUCLEAR ANTIGEN(S) ASSOCIATED WITH CELL PROLIFERATION

An anti-nucleolar antiserium is successfully raised in rabbits. The nature of the nucleolar antigen(s) (NAg-1) is investigated. Indirect immunofluorescent staining and cytochemical analysis suggest that the MAg-1 is an (are) acidic protein(s), complexed with DNA and related to rRNA synthesis. Strong nucleolar fluorescence is observed in human cancer cells and proliferating cells but not in normal human lymphocytes and non-proliferating tissues. The nucleolar antigen(s) is (are) not detected in the cells of mouse. Using human promyelocytic tumor cells line HL-60 it is shown that those with a higher speed of growth possesses higher percentages of NAg-1 positive cells than the group with a slower growth rate. With the increase of cell density the percentage of NAg-1 positive cells decline. These preliminary studies demonstrate that the NAg-1 antigen(s) is (are) associated with cell proliferation.